Valentina Riveros-Moreno

TL;DR: The distribution of the immunoreactivity to nitric oxide synthase has been examined from rostral to caudal areas of the rat central nervous system using light microscopy.

TL;DR: The complete structure of the P195 gene determined from further DNA clones is described, its organization within genomic DNA and the location of the specific processing fragments within the primary amino-acid sequence are described.

TL;DR: Toxic megacolon is associated with the appearance of inducible NO synthase in the Colonic muscularis propria, and local generation of excessive amounts of NO may be responsible for the colonic dilatation that is the hallmark of this syndrome.

TL;DR: The amino acid sequence for the constitutive rat brain nitric oxide (NO) synthase was analysed by a set of computer programs that estimate and display physicochemical properties such as hydrophilicity, flexibility, accessibility, hydrophilic periodicity and conformation to postulate that either the peptides when conjugated to the carrier protein attain a different conformed to that in the native NO synthase, or alternatively the accessibility of the antibodies to substrate binding sites is highly restricted by steric

TL;DR: A substantial proportion of the total NO synthase produced becomes soluble following addition of hemin to the culture medium, and it is demonstrated that the enzyme activity is exclusively associated with the dimeric form of the enzyme, which had the following molar ratios for the cofactors.