Wenqin Ying

TL;DR: A method named GOTI (genome-wide off-target analysis by two-cell embryo injection) is developed to detect off- target mutations by editing one blastomere of two- cell mouse embryos using either CRISPR-Cas9 or base editors.

TL;DR: A homology-mediated end joining (HMEJ)-based strategy is devised, using CRISPR/Cas9-mediated cleavage of both transgene donor vector that contains guide RNA target sites and ∼800 bp of homology arms, and the targeted genome, with an efficiency much greater than HR-, NHEJ- and MMEJ-based strategies.

TL;DR: A transgenic mouse is generated using an improved dCas9 system that enables simultaneous and precise in vivo transcriptional activation of multiple genes and long noncoding RNAs in the nervous system and is able to use targeted activation of endogenous neurogenic genes in these transgenic mice to directly and efficiently convert astrocytes into functional neurons in vivo.

TL;DR: It is shown that single gene or multiple genes can be completely knocked out in mouse and monkey embryos by zygotic injection of Cas9 mRNA and multiple adjacent single-guide RNAs that target only a single key exon of each gene.

TL;DR: CRISPR/Cas9-mediated targeted chromosome elimination offers a new approach to develop animal models with chromosome deletions, and a potential therapeutic strategy for human aneuploidy diseases involving additional chromosomes.