Pengyu Huang

TL;DR: This study demonstrates the direct induction of functional hepatocyte-like (iHep) cells from mouse tail-tip fibroblasts by transduction of Gata4, Hnf1α and Foxa3, and inactivation of p19Arf and provides a novel strategy to generate functional hepatocytes-like cells for the purpose of liver engineering and regenerative medicine.

TL;DR: Upon transplantation into mice with concanavalin-A-induced acute liver failure and fatal metabolic liver disease due to fumarylacetoacetate dehydrolase (Fah) deficiency, hiHeps restore the liver function and prolong survival.

TL;DR: A homology-mediated end joining (HMEJ)-based strategy is devised, using CRISPR/Cas9-mediated cleavage of both transgene donor vector that contains guide RNA target sites and ∼800 bp of homology arms, and the targeted genome, with an efficiency much greater than HR-, NHEJ- and MMEJ-based strategies.

TL;DR: Recent findings that reveal the profound impact ofMitogen-activated protein kinases on chronic inflammation and, particularly, inflammation-associated cancer development are discussed.

TL;DR: A transgenic mouse is generated using an improved dCas9 system that enables simultaneous and precise in vivo transcriptional activation of multiple genes and long noncoding RNAs in the nervous system and is able to use targeted activation of endogenous neurogenic genes in these transgenic mice to directly and efficiently convert astrocytes into functional neurons in vivo.