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William E. Theurkauf
Researcher at University of Massachusetts Medical School
Publications - 88
Citations - 11794
William E. Theurkauf is an academic researcher from University of Massachusetts Medical School. The author has contributed to research in topics: Piwi-interacting RNA & Microtubule. The author has an hindex of 54, co-authored 87 publications receiving 11194 citations. Previous affiliations of William E. Theurkauf include University of California, Santa Cruz & University of California, San Francisco.
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Journal ArticleDOI
Normal microRNA Maturation and Germ-Line Stem Cell Maintenance Requires Loquacious, a Double-Stranded RNA-Binding Domain Protein
Klaus Förstemann,Yukihide Tomari,Tingting Du,Vasily V. Vagin,Ahmet M. Denli,Diana P. Bratu,Carla Andrea Klattenhoff,William E. Theurkauf,Phillip D. Zamore +8 more
TL;DR: It is shown that normal processing of Drosophila pre-miRNAs by Dicer-1 requires the double-stranded RNA-binding domain (dsRBD) protein Loquacious (Loqs), a homolog of human TRBP, a protein first identified as binding the HIV trans-activator RNA (TAR).
Journal ArticleDOI
Collapse of Germline piRNAs in the Absence of Argonaute3 Reveals Somatic piRNAs in Flies
Chengjian Li,Vasily V. Vagin,Soohyun Lee,Soohyun Lee,Jia Xu,Jia Xu,Shengmei Ma,Hualin Xi,Hualin Xi,Hervé Seitz,Michael D. Horwich,Monika Syrzycka,Barry M. Honda,Ellen L. W. Kittler,Maria L. Zapp,Carla Andrea Klattenhoff,Nadine Schulz,William E. Theurkauf,Zhiping Weng,Phillip D. Zamore +19 more
TL;DR: Strong loss-of-function mutations in ago3 are described and it is found that Ago3 acts to amplify piRNA pools and to enforce on them an antisense bias, increasing the number of piRNAs that can act to silence transposons.
Journal ArticleDOI
Biogenesis and germline functions of piRNAs.
TL;DR: Whether piRNAs primarily control chromatin organization, gene transcription, RNA stability or RNA translation is not well understood, neither is piRNA biogenesis, and unanswered questions are discussed.
Journal ArticleDOI
RISC assembly defects in the Drosophila RNAi mutant armitage.
Yukihide Tomari,Tingting Du,Benjamin Haley,Dianne S. Schwarz,Ryan Bennett,Heather A Cook,Birgit S. Koppetsch,William E. Theurkauf,Phillip D. Zamore +8 more
TL;DR: It is shown that armi is required for RNAi, and native gel analysis of protein-siRNA complexes in wild-type and armi mutant ovary lysates suggests that armu mutants support early steps in the RNAi pathway but are defective in the production of active RNA-induced silencing complex (RISC), which mediates target RNA destruction in RNAi.
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Reorganization of the cytoskeleton during Drosophila oogenesis: implications for axis specification and intercellular transport.
TL;DR: This work believes that actin plays a secondary role in each of these morphogenetic events, based on parallel studies of actin organization during each of the above stages of oogenesis.