Beijing Wu

TL;DR: This method probes DNA accessibility with hyperactive Tn5 transposase, which inserts sequencing adapters into accessible regions of chromatin, which can be used to infer regions of increased accessibility, as well as to map regions of transcription‐factor binding and nucleosome position.

TL;DR: A robust method for mapping the accessible genome of individual cells by assay for transposase-accessible chromatin using sequencing (ATAC-seq) integrated into a programmable microfluidics platform is developed and single-cell analysis of DNA accessibility provides new insight into cellular variation of the ‘regulome’.

TL;DR: The Omni-ATAC protocol generates chromatin accessibility profiles from archival frozen tissue samples and 50-μm sections, revealing the activities of disease-associated DNA elements in distinct human brain structures.

TL;DR: These studies identified regions of the nematode and fly genomes that show highly occupied targets (or HOT) regions where DNA was bound by more than 15 of the transcription factors analyzed and the expression of related genes were characterized, providing insights into the organization, structure, and function of the two genomes.

TL;DR: The chromatin accessibility and transcriptional landscapes in 13 human primary blood cell types that span the hematopoietic hierarchy are defined and 'enhancer cytometry' is enabled for enumeration of pure cell types from complex populations.