Food waste generation and industrial uses: A review.

Enhancement of methane production in anaerobic digestion process: A review

Microbial ecology of denitrification in biological wastewater treatment.

Anaerobic digestion of food waste for volatile fatty acids (VFAs) production with different types of inoculum: Effect of pH

Enhanced volatile fatty acids production from anaerobic fermentation of food waste: A mini-review focusing on acidogenic metabolic pathways.

Short-chain fatty acids (SCFAs) have been regarded as the excellent carbon source of wastewater biological nutrient removal, and sludge alkaline (pH 10) fermentation has been reported to achieve highly efficient SCFAs production. In this study, the underlying mechanisms for the improved SCFAs production at pH 10 were investigated by using 454 pyrosequencing and fluorescent in situ hybridization (FISH) to analyze the microbial community structures in sludge fermentation reactors. It was found that sludge fermentation at pH 10 increased the abundances of Pseudomonas sp. and Alcaligenes sp., which were able to excrete extracellular proteases and depolymerases, and thus enhanced the hydrolysis of insoluble sludge protein and polyhydroxyalkanoates (PHA). Meanwhile, the abundance of acid-producing bacteria (such as Clostridium sp.) in the reactor of pH 10 was also higher than that of uncontrolled pH, which benefited the acidification of soluble organic substrates. Further study indicated that sludge fermentatio...

Pyrosequencing reveals the key microorganisms involved in sludge alkaline fermentation for efficient short-chain fatty acids production.

The use of sludge fermentative short-chain fatty acids (SCFA) as an additional carbon source of biological nutrient removal (BNR) has drawn much attention recently as it can reuse sludge organics, reduce waste activated sludge production, and improve BNR performance. Our previous laboratory study had shown that the SCFA production was significantly enhanced by controlling sludge fermentation at pH 10 with NaOH. This paper focused on a pilot-scale study of alkaline fermentation of waste activated sludge, separation of the fermentation liquid from the alkaline fermentation system, and application of the fermentation liquid to improve municipal biological nitrogen and phosphorus removal. NaOH and Ca(OH)(2) were used respectively to adjust the alkaline fermentation pH, and their effects on sludge fermentation and fermentation liquid separation were compared. The results showed that the use of Ca(OH)(2) had almost the same effect on SCFA production improvement and sludge volatile suspended solids reduction as that of NaOH, but it exhibited better sludge dewatering, lower chemical costs, and higher fermentation liquid recovery efficiency. When the fermentation liquids, adjusted with Ca(OH)(2) and NaOH respectively, were added continuously to an anaerobic-anoxic-aerobic municipal wastewater BNR system, both the nitrogen and phosphorus removals, compared with the control, were improved to the same levels. This was attributed to the increase of not only influent COD but also denitrifying phosphorus removal capability. It seems that the use of Ca(OH)(2) to control sludge fermentation at pH 10 for efficiently producing a carbon source for BNR is feasible.

Pilot-scale waste activated sludge alkaline fermentation, fermentation liquid separation, and application of fermentation liquid to improve biological nutrient removal.

Magnetic biochar catalyst derived from biological sludge and ferric sludge using hydrothermal carbonization: Preparation, characterization and its circulation in Fenton process for dyeing wastewater treatment

https://escholarship.org/content/qt3zr273fm/qt3zr273fm.pdf?t=ok16jc

Comparative analysis of energy intensity and carbon emissions in wastewater treatment in USA, Germany, China and South Africa

Simultaneous nitrification and denitrification (SND) is a promising single-reactor biological nitrogen-removal method. Activated sludge with and without iron scrap supplementation (Sludge-Fe and Sludge-C, respectively) was acclimated under aerobic condition. The total nitrogen (TN) content of Sludge-Fe substantially decreased from 25.0 ± 1.0 to 11.2 ± 0.4 mg/L, but Sludge-C did not show the TN-removal capacity. Further investigations excluded a chemical reduction of NO3--N by iron and a decrease of NH4+-N by microbial assimilation, and the contribution of SND was verified. Moreover, the amount of aerobic denitrifiers, such as bacteria belonging to the genera Thauera, Thermomonas, Rhodobacter, and Hyphomicrobium, was considerably enhanced, as observed through Miseq Illumina sequencing method. The activities of the key enzymes ammonia monooxygenase (AMO) and nitrite oxidoreductase (NXR), which are associated with nitrification, and periplasmic nitrate reductase (NAP) and nitrite reductase (NIR), which are related to denitrification, in Sludge-Fe were 1.23-, 1.53-, 3.60-, and 1.55-fold higher than those in Sludge-C, respectively. In Sludge-Fe, the quantity of the functional gene NapA encoding enzyme NAP, which is essential for aerobic denitrification, was significantly promoted. The findings indicate that SND is the primary mechanism underlying the removal of TN and that iron scrap can robustly stimulate SND under aerobic environment.

Xiang Li

Papers

Pyrosequencing reveals the key microorganisms involved in sludge alkaline fermentation for efficient short-chain fatty acids production.

Pilot-scale waste activated sludge alkaline fermentation, fermentation liquid separation, and application of fermentation liquid to improve biological nutrient removal.

Magnetic biochar catalyst derived from biological sludge and ferric sludge using hydrothermal carbonization: Preparation, characterization and its circulation in Fenton process for dyeing wastewater treatment

Comparative analysis of energy intensity and carbon emissions in wastewater treatment in USA, Germany, China and South Africa

Iron Robustly Stimulates Simultaneous Nitrification and Denitrification Under Aerobic Conditions.