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Xueyan Li
Researcher at Chinese Academy of Sciences
Publications - 5
Citations - 789
Xueyan Li is an academic researcher from Chinese Academy of Sciences. The author has contributed to research in topics: RNA & Trans-activating crRNA. The author has an hindex of 5, co-authored 5 publications receiving 512 citations.
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Journal ArticleDOI
The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a.
Liang Liu,Xueyan Li,Jun Ma,Zongqiang Li,Lilan You,Jiuyu Wang,Min Wang,Xinzheng Zhang,Yanli Wang +8 more
TL;DR: Findings reveal how Cas13a of type VI CRISPR-Cas systems defend against RNA phages and set the stage for its development as a tool for RNA manipulation.
Journal ArticleDOI
Two Distant Catalytic Sites Are Responsible for C2c2 RNase Activities
TL;DR: The structures of Leptotrichia shahii C2c2 in its crRNA-free and cr RNA-bound states are reported to provide important insights into the molecular mechanism of dual RNase activities of C 2c2 and establish a framework for its future engineering as a RNA editing tool.
Journal ArticleDOI
C2c1-sgRNA Complex Structure Reveals RNA-Guided DNA Cleavage Mechanism
TL;DR: The findings indicate that sgRNA assembly is achieved through a mechanism distinct from that reported previously for Cas9 or Cpf1 endonucleases, which renders C2c1 a valuable addition to the current arsenal of genome-editing tools.
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A CRISPR-Cas13a system for efficient and specific therapeutic targeting of mutant KRAS for pancreatic cancer treatment
Xiao Zhao,Liang Liu,Jiayan Lang,Keman Cheng,Yongwei Wang,Xueyan Li,Jian Shi,Yanli Wang,Guangjun Nie,Guangjun Nie +9 more
TL;DR: It is demonstrated that the CRISPR-Cas13a system can be engineered for targeted therapy of mutant KRAS in pancreatic cancer and an optimization strategy for the development of a CRISpr-Cas 13a system to affect efficient and specific knockdown of the oncogenic mRNA is described.
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Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2.
Xue Huang,Wei Sun,Zhi Cheng,Minxuan Chen,Xueyan Li,Jiuyu Wang,Gang Sheng,Weimin Gong,Yanli Wang +8 more
TL;DR: The mechanism of DNA recognition and cleavage by Cas12i2, and activation of the RuvC catalytic pocket induced by a conformational change of the Helical-II domain are revealed, providing significant insights into the DNA cleavage mechanism by Ruv C-containing Cas proteins.