Yannick Bellec

TL;DR: It is demonstrated by in vivo bimolecular fluorescence complementation that PAS2 was specifically associated in the endoplasmic reticulum with the enoyl-CoA reductase CER10, the fourth enzyme of the elongase complex, indicating premature termination of fatty acid elongation and confirming the role of PAS 2 in this process.

TL;DR: Selective, targeted mutagenesis of the three delta‐12‐desaturase (FAD2) genes was achieved by CRISPR‐Cas9 gene editing, leading to reduced levels of polyunsaturated fatty acids and increased accumulation of oleic acid in the oil.

TL;DR: It is shown that Arabidopsis has two classes of ceramide synthases discriminating acyl chain length and also that very-long-acyl-chain sphingolipids are required for polar auxin transport in particular during lateral root emergence and a trafficking pathway with specific endomembrane compartments and polar auxIn transport protein cargoes is defined.

TL;DR: This work presents an efficient in planta transient transformation system that allows single or multiple expression of constructs containing various fluorescent protein tags in Arabidopsis cotyledons, and uses this system to investigate the subcellular distribution of enzymes involved in sphingolipid metabolism.

TL;DR: It is proposed that PAS1 acts as a molecular scaffold for the fatty acid elongase complex in the endoplasmic reticulum and that the resulting VLCFAs are required for polar auxin transport and tissue patterning during plant development.