Zeyao Li

TL;DR: Three widely used droplet-based ultra-high-throughput single-cell RNA-seq systems, inDrop, Drop-seq, and 10X Genomics Chromium are developed and detailed comparison revealed the distinguishing features and suitable applications for each system.

TL;DR: It is found that null mutants in either gene FEN1 or FEN12 exhibit resistance to fluconazole, a first-line antifungal drug, and the new transposon-mediated mutagenesis system should facilitate future genome-wide studies of C. albicans.

TL;DR: A light-activated RNA labeling method was developed to determine spatial organization of a transcriptome and found that ribosomal proteins and oxidative phosphorylation pathway proteins are highly enriched at the outer mitochondrial membrane.

TL;DR: APEX2-mediated Btn-An labeling of RNA is thus a promising method for mapping the subcellular transcriptome, which could shed light on their functions in cell physiology.

TL;DR: It is shown that pancreatic inflammation is coupled to the emergence of a transient progenitor cell population that is readily transformed in the presence of mutant KrasG12D, providing an oncogenic transcriptional program that fuels tumor progression beyond initiation, accounting for the intra-tissue selectivity of Kras transformation.