Leonard J. Foster

TL;DR: This work developed PSORTb version 3.0 with improved recall, higher proteome-scale prediction coverage, and new refined localization subcategories, and evaluated the most accurate SCL predictors using 5-fold cross validation plus an independent proteomics analysis.

TL;DR: This work uses quantitative high-resolution MS to specifically detect proteins depleted from rafts by cholesterol-disrupting drugs, resulting in a set of 241 authentic lipid raft components, providing the first large-scale and unbiased evidence for the connection of rafts with signaling and place limits on the fraction of plasma membrane composed by rafts.

TL;DR: Stable isotopic amino acids in cell culture is employed to differentially label proteins in EGF-stimulated versus unstimulated cells and SILAC combined with modification-based affinity purification is a useful approach to detect specific and functional protein-protein interactions.

TL;DR: This analysis ties biochemistry, cell biology, and genomics into a common framework for organelle analysis and identifies networks of coexpressed genes, cis-regulatory motifs, and putative transcriptional regulators involved in organelle biogenesis.

TL;DR: The approach, named terminal amine isotopic labeling of substrates (TAILS), addresses this challenge by using dendritic polyglycerol aldehyde polymers that remove tryptic and C-terminal peptides to discriminate between the substrates of the protease of interest and the products of background proteolysis.