Showing papers by "Central Drug Research Institute published in 1972"
TL;DR: In this article, the aporphine alkaloids anonaine, roemerine, norcorydine and glaucine have been isolated from Annona squamosa.
63 citations
57 citations
TL;DR: Evidence is adduced in this communication to show that cyclic 3', 5'-adenosine monophosphate (CAMP) can replace magnesium ions and taurine in the nonnutrient agar medium and that CAMP brings about a comparable degree of encystation of the ameba.
Abstract: Hartmannella culbertsoni, a free-living soil ameba, undergoes differentiation to be transformed into a double-walled cyst when exposed to a nonnutrient medium containing magnesium ions and taurine (Raizada and Krishna Murti, 1971) . The transformation of the trophic form to mature cysts is usually preceded by a stage during induction when the ameba assumes a round and well-defined shape after the withdrawal of the pseudopodia. Evidence is adduced in this communication to show that cyclic 3', 5'-adenosine monophosphate (CAMP) can replace magnesium ions and taurine in the nonnutrient agar medium and that CAMP brings about a comparable degree of encystation of the ameba. The presence of magnesium ions and taurine has also been shown to result in a threeto fourfold increase in the rate of synthesis of CAMP by the ameba .
37 citations
TL;DR: The aporphine alkaloids, norboldine, boldine, laurotetanine, N-methyllaurotetsanine and actinodaphnine have been isolated from Litsea glutenosa var. glabraria Hook as discussed by the authors.
31 citations
27 citations
TL;DR: Asclepin has been shown to be 3′-O-acetylcalotropin using various chemical and physical methods as mentioned in this paper, and the NMR data of all the Calotropis glycosides have been analysed in conjunction with that of asclepin.
26 citations
TL;DR: Boldine, laurotetanine, N -methyllaurotenine, and actinodaphnine from Litsea sebifera pers.
22 citations
17 citations
TL;DR: In this paper, various spectroscopic and chemical methods have been employed to derive the structure of carindone as a novel type of C 31 -terpenoid, which was then used to derive its structure.
16 citations
TL;DR: The adrenergic neurones appear to have an excitatory function in central cardiovascular regulation as well as direct, excitability of vasomotor loci in anaesthetized cats.
15 citations
TL;DR: Quantitative changes in the contents of protein, free amino acids, starch, polyfructosans, soluble sugars, phosphates and the activities of ten enzymes during 7 days of germination of Cicer arietinum (chick pea: Bengal gram) are reported.
TL;DR: In this article, the Synthese vondl-O-Methylmahanine wird beschrieben und damit die Struktur (IIa) fur Mahanine bewiesen.
Abstract: Die Synthese vondl-O-Methylmahanine wird beschrieben und damit die Struktur (IIa) fur Mahanine bewiesen.
TL;DR: CMC appears to be a suitable carrier-vehicle for instillation of quinacrine and in the aqueous-quinacrine treated rats and also in the cases with ligation there was an extensive nonlocalized effect.
TL;DR: Hartmannella culbertsoni trophozoites are transformed into viable cysts on exposure to a non-nutrient agar medium containing 15 mM MgCl 2 and 20 mM taurine.
Abstract: Hartmannella culbertsoni trophozoites are transformed into viable cysts on exposure to a non-nutrient agar medium containing 15 mM MgCl 2 and 20 mM taurine. Amebae differentiating in this encystment medium incorporate more uracil-2- 14 C into RNA and more leucine-1- 14 C or valine-1- 14 C into proteins than controls. Encysting organisms incorporate significantly more glucose-U- 14 C into cellulose and glucosamine-1- 14 C into mucopolysaccharides. Incorporation of glucose-U- 14 C into cellulose and of glucosamine-1- 14 C into mucopolysaccharides are inhibited by actinomycin D or cycloheximide.
TL;DR: When seeds were exposed to inhibitors during imbibition, the induction of proteases, amylase, phosphatase and peroxidase activities that occurred during germination was inhibited, indicating that these enzymes appeared in seedlings by de dovo synthesis.
TL;DR: In this paper, the exact configurations of crotsparine, sparsiflorine, and sparsiferine have been determined, and the exact configuration of sparsify has been determined.
TL;DR: Catalase activity was significantly stimulated by the copper device as compared to that by the nylon suture, and peroxidase and cytochrome c oxidase activities did not show any noteworthy change.
TL;DR: Excystation of Schizopyrenus russelli was associated with increase in DNA, RNA, and protein and both excystment and these macromolecular increases were sensitive to ultraviolet radiation.
Abstract: SYNOPSIS. Excystment of Schizopyrenus russelli was associated with increase in DNA, RNA, and protein. Both excystment and these macromolecular increases were sensitive to ultraviolet radiation. The effect of UV was partly reversed by incubation in the dark at 28 C, but not at 4 C. Excystment from cysts preincubated in the excystment medium (before UV treatment) was relatively resistant to radiation.
TL;DR: Racemic O,O′-dibenzylisococlaurine has been resolved and the absolute configuration of (+) and (−) isococlaine determined.
TL;DR: Glycine antagonized strychnine‐induced facilitation of FR, CER and LMR and was 5–6 times as active as γ‐aminobutyric acid in this respect, and the possibility of glycine being a transmitter in spinal pathways is discussed.
Abstract: 1. The effects of intracerebroventricular or intrathecal administration of glycine on some somatic reflexes in chloralosed cats were investigated.
2. Glycine produced marked inhibition of the flexor reflex (FR) and crossed extensor reflex (CER) at low doses (2·5-5·0 mg) but even large doses (up to 40·0 mg) had no effect on the facilitation or inhibition of the patellar reflex produced by stimulation of either the reticular formation or the sciatic nerve. The linguomandibular reflex (LMR) could be blocked by 10-20 mg glycine.
3. Glycine antagonized strychnine-induced facilitation of FR, CER and LMR and was 5-6 times as active as γ-aminobutyric acid in this respect.
4. The possibility of glycine being a transmitter in spinal pathways is discussed.
TL;DR: Sodium lauryl sulphate had a marked inhibitory effect on the SDH activity of the mitochondrial suspension and together with Emetine it counteracted the activation caused by emetine alone, and Lysozyme had little effect in such experiments.
TL;DR: Serologic reactions elicited by sonically ruptured trophic and cystic forms of Hartmannella culbertsoni were studied and non-specific complement fixation mediated by cyst antigens was abolished by treatment with cellulase.
Abstract: SYNOPSIS. Serologic reactions elicited by sonically ruptured trophic and cystic forms of Hartmannella culbertsoni were studied. The antigens of trophic amoebae reacted with their homologous rabbit antiserum showing multiple precipitin lines which could not be seen when the reacting antigens were treated with trypsin prior to application on the Ouchterlony plates. Antigens of trophic amoeba did not react with antiserum against cysts. Cyst antigens reacted with their homologous antiserum only after trypsin treatment. Antigens prepared from trophozoites excysting from cysts reacted positively with the antiserum against antigens of trophic amoebae.
Antigens of trophic as well as cystic forms fixed guinea pig complement in presence of their homologous antisera. With the trophic form, this property was abolished after trypsin treatment. Non-specific complement fixation mediated by cyst antigens was abolished by treatment with cellulase. Antiserum against trophic amoebae immobilized trophozoites and, in the presence of guinea pig complement, led to their lysis.
TL;DR: In this article, an improved microcombustion technique for nitrogen estimation in heterocyclic compounds by use of a modified Dumas filling is described, which is based on a modified version of the Dumas mixture.
Abstract: An improved microcombustion technique is described for nitrogen estimation in heterocyclic compounds by use of a modified Dumas filling.
TL;DR: Increases in the cholesterol levels of liver mitochondria) and microsomal fractions was observed in all the groups and appeared to be directly correlated with the atheroma index.
TL;DR: It appears from the present work that 4 to 5 days post partum the Fallopian tube has a higher number of estradiol receptors than the tube during the follicular phase of the cycle and during prolonged lactational amenorrhea, which may be related in part to the endogenous estrogen level.
TL;DR: Activity in the cell-free extracts of the trophozoites of Hartmannella culbertsoni has been purified 20-fold, indicating allosteric nature of the enzyme.
Abstract: 0730 09
1.
l-Threonine dehydratase (E.C. 4.2.1.16) activity in the cell-free extracts of the trophozoites of Hartmannella culbertsoni has been purified 20-fold.
2.
The enzyme requires pyridoxal-5-phosphate for its activity and is not cold-labile. Kinetic studies have shown that there is homotropic cooperative interaction between the substrate molecules. The enzyme is susceptible to end-product regulation, l-isoleucine being a feed back inhibitor and l-valine can partially reactivate the l-isoleucine inhibited enzyme. Some nucleotides stimulate the activity of the enzyme indicating allosteric nature of the enzyme.
3.
Certain amoebicidal drugs and antibiotics have marked inhibitory effect on the enzyme.