Kyonggi University

About: Kyonggi University is a education organization based out in Suwon, South Korea. It is known for research contribution in the topics: Catalysis & Dielectric. The organization has 1946 authors who have published 4404 publications receiving 64791 citations.

Practical Experiences and Requirements on Workflow

Abstract: The purposes of this paper are to describe practical experiences on a business process(Hiring Process) automation by a workflow automation approach, and to point out the limitations of current business process automation from the workflow application developer's point of view. The limitations may be caused from the lack of human coordination, collaboration, group-decision, interoperability, scalability, correctness/reliability of workflow applications, exception handling, and a dynamic reflex of the organizational changes in current workflow modeling methodologies and workflow management systems.

Regulator of G protein signaling 2 (RGS2) and RGS4 form distinct G protein-dependent complexes with protease activated-receptor 1 (PAR1) in live cells.

Abstract: Protease-activated receptor 1 (PAR1) is a G-protein coupled receptor (GPCR) that is activated by natural proteases to regulate many physiological actions. We previously reported that PAR1 couples to Gi, Gq and G12 to activate linked signaling pathways. Regulators of G protein signaling (RGS) proteins serve as GTPase activating proteins to inhibit GPCR/G protein signaling. Some RGS proteins interact directly with certain GPCRs to modulate their signals, though cellular mechanisms dictating selective RGS/GPCR coupling are poorly understood. Here, using bioluminescence resonance energy transfer (BRET), we tested whether RGS2 and RGS4 bind to PAR1 in live COS-7 cells to regulate PAR1/Gα-mediated signaling. We report that PAR1 selectively interacts with either RGS2 or RGS4 in a G protein-dependent manner. Very little BRET activity is observed between PAR1-Venus (PAR1-Ven) and either RGS2-Luciferase (RGS2-Luc) or RGS4-Luc in the absence of Gα. However, in the presence of specific Gα subunits, BRET activity was markedly enhanced between PAR1-RGS2 by Gαq/11, and PAR1-RGS4 by Gαo, but not by other Gα subunits. Gαq/11-YFP/RGS2-Luc BRET activity is promoted by PAR1 and is markedly enhanced by agonist (TFLLR) stimulation. However, PAR1-Ven/RGS-Luc BRET activity was blocked by a PAR1 mutant (R205A) that eliminates PAR1-Gq/11 coupling. The purified intracellular third loop of PAR1 binds directly to purified His-RGS2 or His-RGS4. In cells, RGS2 and RGS4 inhibited PAR1/Gα-mediated calcium and MAPK/ERK signaling, respectively, but not RhoA signaling. Our findings indicate that RGS2 and RGS4 interact directly with PAR1 in Gα-dependent manner to modulate PAR1/Gα-mediated signaling, and highlight a cellular mechanism for selective GPCR/G protein/RGS coupling.

Enhancement of self-healing property by introducing ethylene glycol group into thermally reversible Diels-Alder reaction based self-healable materials

Abstract: Herein, the effect of chain mobility on the efficiency of self-healing was investigated based on thermally reversible Diels-Alder reaction. Ethylene glycol group was chosen as the functional group for increasing chain mobility of the furan functionalized polymethacrylate and bismaleimide, respectively. From the thermal analysis of the films prepared with various combinations between furan functionalized polymethacrylates and bismaleimide, it was found that glass transition temperature of films decreased with increasing the content of ethylene glycol group. Comparing the state of film before and after the self-healing process by optical microscope images, it was also confirmed that the film prepared with polymer and bismaleimide having high ratio of ethylene glycol group (FEEMA55 and bismaleimide-1) has high self-healing efficiency. Therefore, the improving mobility by the introduction of ethylene glycol group plays an important role for the enhancement of self-healing property.

Biodegradation of diesel oil and n-alkanes (C 18 , C 20 , and C 22 ) by a novel strain Acinetobacter sp. K-6 in unsaturated soil

Abstract: A large residual fraction of aliphatic components of diesel prevails in soil, which has adverse effects on the environment. This study identified the most bio-recalcitrant aliphatic residual fraction of diesel through total petroleum-hydrocarbon fractional analysis. For this, the strain Acinetobacter sp. K-6 was isolated, identified, and characterized and investigated its ability to degrade diesel and n-alkanes (C18, C20, and C22). The removal efficiency was analysed after treatment with bacteria and nutrients in various soil microcosms. The fractional analysis of diesel degradation after treatment with the bacterial strains identified C18-C22 hydrocarbons as the most bio-recalcitrant aliphatic fraction of diesel oil. Acinetobacter sp. K-6 degraded 59.2% of diesel oil and 56.4% of C18-C22 hydrocarbons in the contaminated soil. The degradation efficiency was further improved using a combinatorial approach of biostimulation and bioaugmentation, which resulted in 76.7% and 73.7% higher degradation of diesel oil and C18-C22 hydrocarbons, respectively. The findings of this study suggest that the removal of mid-length, non-volatile hydrocarbons is affected by the population of bio-degraders and the nutrients used in the process of remediation. A combinatorial approach, including biostimulation and bioaugmentation, could be used to effectively remove large quantities of aliphatic hydrocarbons persisting for a longer period in the soil.