Institution
Mannheim University of Applied Sciences
Education•Mannheim, Germany•
About: Mannheim University of Applied Sciences is a education organization based out in Mannheim, Germany. It is known for research contribution in the topics: Gene & Mass spectrometry imaging. The organization has 593 authors who have published 779 publications receiving 19248 citations. The organization is also known as: FH Mannheim & Mannheim College.
Papers published on a yearly basis
Papers
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TL;DR: This work shows that an Arabidopsis 2-oxoglutarate–dependent dioxygenase, an activity associated with histone demethylation in other organisms, physically associates with the Polycomb Repressive Complex 2 and proposes that physical association of histone methylation/demethylation activities will be generally important to coordinate switching between chromatin states.
Abstract: Molecular mechanisms enabling the switching and maintenance of epigenetic states are not fully understood. Distinct histone modifications are often associated with ON/OFF epigenetic states, but how these states are stably maintained through DNA replication, yet in certain situations switch from one to another remains unclear. Here, we address this problem through identification of Arabidopsis INCURVATA11 (ICU11) as a Polycomb Repressive Complex 2 accessory protein. ICU11 robustly immunoprecipitated in vivo with PRC2 core components and the accessory proteins, EMBRYONIC FLOWER 1 (EMF1), LIKE HETEROCHROMATIN PROTEIN1 (LHP1), and TELOMERE_REPEAT_BINDING FACTORS (TRBs). ICU11 encodes a 2-oxoglutarate-dependent dioxygenase, an activity associated with histone demethylation in other organisms, and mutant plants show defects in multiple aspects of the Arabidopsis epigenome. To investigate its primary molecular function we identified the Arabidopsis FLOWERING LOCUS C (FLC) as a direct target and found icu11 disrupted the cold-induced, Polycomb-mediated silencing underlying vernalization. icu11 prevented reduction in H3K36me3 levels normally seen during the early cold phase, supporting a role for ICU11 in H3K36me3 demethylation. This was coincident with an attenuation of H3K27me3 at the internal nucleation site in FLC, and reduction in H3K27me3 levels across the body of the gene after plants were returned to the warm. Thus, ICU11 is required for the cold-induced epigenetic switching between the mutually exclusive chromatin states at FLC, from the active H3K36me3 state to the silenced H3K27me3 state. These data support the importance of physical coupling of histone modification activities to promote epigenetic switching between opposing chromatin states.
20 citations
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TL;DR: This work reviews the state-of-the-art in image-based in situ methods with regard to their potential use for fermentation of Saccharomyces cerevisiae in sugarcane wine and reagent-free methods that estimate viability from information contained in micrograph images are reviewed.
20 citations
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TL;DR: In this article, the pore size distributions of the samples were measured by mercury intrusion porosimetry and used to calculate the gas thermal conductivity as a function of the residual pressure.
20 citations
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TL;DR: This study demonstrates that different tissues, spray-coated with substance P as a tracer, digest this peptide with different time-course profiles and reveals that distinct cleavage products originating from substance P are generated transiently and that proteolysis can be attenuated by proteases in a concentration-dependent manner.
20 citations
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TL;DR: In this paper, an approach to obtain and model kinetic data by combining a microreactor setup and real-time reaction monitoring through inline Fourier transform infrared spectroscope was presented.
20 citations
Authors
Showing all 597 results
Name | H-index | Papers | Citations |
---|---|---|---|
Ronald R. Breaker | 98 | 257 | 34496 |
Norbert Gretz | 60 | 343 | 11308 |
Michael Christ | 50 | 227 | 8653 |
Martin Wehling | 50 | 265 | 9771 |
Carsten Sticht | 40 | 166 | 6030 |
Carsten Hopf | 35 | 123 | 7121 |
Dirk Breitkreutz | 35 | 71 | 7334 |
Roger Sandhoff | 32 | 76 | 3603 |
Ivo Wolf | 30 | 155 | 4636 |
Mathias Hafner | 29 | 93 | 3273 |
Petra Kioschis | 28 | 54 | 5447 |
Tobias Werner | 27 | 66 | 1941 |
Ralf Lösel | 26 | 38 | 3220 |
Rüdiger Rudolf | 25 | 72 | 2914 |
Martin Feuring | 25 | 77 | 5042 |