Rotterdam University of Applied Sciences

About: Rotterdam University of Applied Sciences is a education organization based out in Rotterdam, Netherlands. It is known for research contribution in the topics: Health care & Population. The organization has 937 authors who have published 1113 publications receiving 27447 citations.

Kinetics and Structure of a Cold-Adapted Hetero-Octameric ATP Phosphoribosyltransferase.

Abstract: Adenosine 5′-triphosphate phosphoribosyltransferase (ATPPRT) catalyzes the first step in histidine biosynthesis, the condensation of ATP and 5-phospho-α-d-ribosyl-1-pyrophosphate to generate N1-(5-phospho-β-d-ribosyl)-ATP and inorganic pyrophosphate. The enzyme is allosterically inhibited by histidine. Two forms of ATPPRT, encoded by the hisG gene, exist in nature, depending on the species. The long form, HisGL, is a single polypeptide chain with catalytic and regulatory domains. The short form, HisGS, lacks a regulatory domain and cannot bind histidine. HisGS instead is found in complex with a regulatory protein, HisZ, constituting the ATPPRT holoenzyme. HisZ triggers HisGS catalytic activity while rendering it sensitive to allosteric inhibition by histidine. Until recently, HisGS was thought to be catalytically inactive without HisZ. Here, recombinant HisGS and HisZ from the psychrophilic bacterium Psychrobacter arcticus were independently overexpressed and purified. The crystal structure of P. arcticus...

Smart Method for Self-Organization in Last-Mile Parcel Delivery

Abstract: Parcel delivery operators experience an increasing pressure to meet the strongly growing demand for delivery services, while protecting city livability and the environment. Improving the performanc...

The geography of container port choice: modelling the impact of hinterland changes on port choice

Abstract: European container ports compete in partially overlapping hinterland areas. The objective of this study is to model port choice and obtain insight into port choice decisions for European container imports from Asia. The importance of port choice factors and their impact on port market shares in the hinterland were investigated. Furthermore, sensitivity of the model in predicting the impact of increasing fuel prices on port hinterlands was tested. Containerised imports of 231 European mainland regions were compiled, based on shipping data, port statistics, modal split and gross regional products. Using literature sources, 11 port choice factors were selected; five of these were found to be statistically significant. These factors and their respective weights were used as input for a logit port choice model to analyse container port imports for 31 ports; the most detailed model yet. A varying oil price scenario was used to show the application and sensitivity of the model. Changing oil prices were found to have an impact on modal split and on the average hinterland transport distance.