Showing papers by "Rush University Medical Center published in 1971"
TL;DR: The isolated protein was found to be homogeneous by ultracentrifugation, free diffusion, and immunochemical criteria, and divalent cations such as Ca++ and Mg++ activated the myosin ATPase activity, whereas EDTA inhibited the enzyme activity.
102 citations
TL;DR: Experimental and clinical evidence is consistent with the hypothesis that aplastic disease of the marrow in human patients may result from immunological damage to the sinusoidal microcirculation, which would explain the failure of isogeneic transplants to repopulate the marrow of patients with aplastics an˦mia.
83 citations
TL;DR: Common side effects encountered were hyperthermic reactions, gastrointestinal disturbances, hyperkeratosis and vesiculation of fingers, alopecia, and stomatitis.
Abstract: Bleomycin is an antitumor antibiotic produced by Streptomyces verticillus. Seventy-five patients with various neoplasms were studied using this drug. Fourteen out of 20 patients with epidermoid carcinoma of the head and neck region, 5 out of 14 cases of lymphoma including Hodgkin's disease, 3 out of 6 patients with testicular tumors, and one patient with lymphangiosarcoma of the arm showed evidence of objective regression. Common side effects encountered were hyperthermic reactions, gastrointestinal disturbances, hyperkeratosis and vesiculation of fingers, alopecia, and stomatitis. Pulmonary fibrosis is a rare but serious complication. One patient in this series died of this complication. There was no evidence of bone marrow, liver, or renal toxicity. Bleomycin promises to be a useful therapeutic agent and merits further study.
67 citations
TL;DR: This observation supports the concept that tardive dyskinesias are related to hypersensitivity of dopaminergic receptors to dopamine and is suggested that Levodopa should be avoided in patients with neuroleptic-induced lingual-facial-buccal Dyskinesia.
63 citations
TL;DR: The data suggest that sodium diatrizoate has a negative inotropic effect on left ventricular myocardium by the third cardiac cycle after injection, and this should be derived from the first 2 cardiac cycles after opacification.
Abstract: Seven selective left ventricular angiocardiograms that permitted measurement of end-diastolic volume for 5 consecutive cardiac cycles were analyzed. Neither end-diastolic volume nor end-systolic volume changed significantly between the first and second cycles after ventricular opacification. A progressive increase in the major and minor ventricular semiaxes occurred beginning with the third opacified cycle. Correspondingly, the average end-diastolic volume and end-systolic volume increased by 6.9 ml and 3.4 ml, respectively, between the second and third cardiac cycles. Ventricular volumes progressively increased between the third and fifth cardiac cycles in each patient. These data suggest that sodium diatrizoate has a negative inotropic effect on left ventricular myocardium by the third cardiac cycle after injection. Physiologic information from cineangiocardiography should be derived from the first 2 cardiac cycles after opacification.
59 citations
TL;DR: An intravenous bolus injection of mithramycin was given to patients with malignancy‐related hypercalcemia persisting after hydmtion, and in most instances a gradual but steady fall in the serum calcium occurred within the next 48 hours, accompanied by a fall in serum inorganic phosphate and 24 hour urinary calcium excretion.
Abstract: An intravenous bolus injection of mithramycin (25 μg per kilogram) was given to patients with malignancy‐related hypercalcemia persisting after hydmtion. In most instances a gradual but steady fall in the serum calcium occurred within the next 48 hours, accompanied by a fall in serum inorganic phosphate and 24 hour urinary calcium excretion. Patients with head and neck cancer, cancer of the lung, and multiple myeloma were somewhat less responsive as a group than patients with cancer of the breast or kidney. Aside from transient nausea and vomiting, side effects were minimal. Small doses of mithramycin inhibit bone resorption; dactinomycin and other RNA inhibitors block the action of vitamin D in vitro. The hypocalcemia following administration of these agents may be an expression of their antivitamin D action.
50 citations
49 citations
TL;DR: The localization of chick embryonic lysozyme was determined by studying the rate of release from the tissue during sequential enzymatic digestion and by immunocytochemistry, indicating that, in this tissue, ly sozyme is primarily extra-cellular.
Abstract: The localization of chick embryonic lysozyme was determined by two techniques: by studying the rate of release from the tissue during sequential enzymatic digestion and by immunocytochemistry. Both techniques indicate that, in this tissue, lysozyme is primarily extra-cellular. Cartilage lysozyme was isolated and partially characterized and found to be identical with egg white lysozyme in its immunologic and enzymatic behavior. In addition, a method for the isolation of large numbers of viable chondrocytes is described.
41 citations
34 citations
TL;DR: The finding of normal cerebrospinal fluid homovanillic acid levels supports the hypothesis that dopamine receptor site hypersensitivity is the primary defect in the initiation of dopamine-induced chorea.
32 citations
TL;DR: Left ventricular subendocardial hemorrhagic necrosis, a lesion different from myocardial infarction, has been considered a major cause of death in patients undergoing operation for valvular heart disease.
TL;DR: An impaired response to both PHA and tumour cells was noted in patients with florid tumours, a form thought to be associated with a diminished host response.
Abstract: This study describes a correlation between the morphologic types of cutaneous Kaposi's sarcomata and the capacity of lymphocytes to be stimulated in culture with PHA and tumour cells. Lymphocytes from patients with the sarcoma and from normal controls were cultured in vitro with PHA, autologous Mitomycin-inhibited tumour cells, and control cells. Lymphocyte transformation was evaluated by measuring the uptake of 125 iodo-2′ deoxyuridine. Lymphocytes from seven of 21 Kaposi patients showed increased transformation in cultures containing tumour cells and six of these seven patients had nodular or infiltrative disease. An impaired response to both PHA and tumour cells was noted in patients with florid tumours, a form thought to be associated with a diminished host response.
Standardized extracts of tumour and control cells were incubated with homologous lymphocytes from other Kaposi patients and normal controls. Two of nine cultures from Kaposi patients showed increased isotope uptake, compared with none of three control cultures.
La presente etude met en evidence une correlation entre les types morphologiques du sarcome cutane de Kaposi et l'aptitude des lymphocytes a se transformer dans des cultures contenant de la PHA et des cellules tumorales. Des lymphocytes de malades atteints de sarcome et de temoins normaux ont ete cultives in vitro avec de la PHA, des cellules-temoins et des cellules tumorales autologues inhibees par la Mitomycine. La transformation des lymphocytes a ete evaluee d'apres l'incorporation de 125 iodo-2' desoxy-uridine. On a constate une transformation accrue des lymphocytes de 7 malades sur 21 dans les cultures contenant des cellules tumorales, dont 6 provenaient de sujets atteints d'une forme de tumeur nodulaire ou infiltrante. On a observe une reponse attenuee a la PHA et aux cellules tumorales chez les malades atteints de tumeurs florides, forme que l'on croit associee a une reponse attenuee de l'hǒte.
Des extraits normalises de cellules tumorales et de cellules-temoins ont ete incubes avec des lymphocytes homologues provenant d'autres sujets atteints de sarcome de Kaposi et de temoins normaux. On a observe une incorporation accrue d'isotopes dans 2 des 9 cultures provenant des malades, ce qui ne s'est produit dans aucune des 3 cultures-temoins.
TL;DR: A patient is described who developed hepatocellular jaundice after methyldopa was administered, and this exacerbation, along with transient arthritis, antinuclear antibodies and positive LE cell tests, document this as a case of hepatotoxicity due to methYldopa.
Abstract: A patient is described who developed hepatocellular jaundice after methyldopa was administered. When the drug was again prescribed, hepatitis recurred. This exacerbation, along with transient arthritis, antinuclear antibodies and positive LE cell tests, document this as a case of hepatotoxicity due to methyldopa.
TL;DR: The data presented here are compatible with the concept that CY somehow improves the environment for hematopoietic stem cell (HSC) proliferation and thereby causes an increased rate of regeneration of transplanted HSC in the marrow cavity of the host.
TL;DR: Lysozyme, a particulate-associated enzyme in other tissues, was not detected in any subcellular fraction even by the sensitive technique of microzone electrophoresis and is therefore considered to be primarily extracellular in cartilage.
Abstract: Some characteristic enzymatic activities were determined in chick embryonic cartilage and compared with the analogous activities in bone and liver. Chondrocytes were isolated, broken by sonication, and subjected to subcellular fractionation to yield a nuclear pellet, the mitochondrial, lysosomal, and microsomal fractions, and the high speed supernatant solution. It was established that these fractions are characterized by enzymatic activities usually associated with similar fractions in other tissues, but with some quantitative differences. Lysozyme, a particulate-associated enzyme in other tissues, was not detected in any subcellular fraction even by the sensitive technique of microzone electrophoresis and is therefore considered to be primarily extracellular in cartilage.
TL;DR: A patient who had severe acquired hypogammaglobulinemia, carcinoma in situ of the stomach, malabsorption syndrome, prolonged Vibrio fetus bacteremia, recurrent thrombophlebitis and lung abscess is presented.
Journal Article•
TL;DR: The linear-nonlinear-linear transition occurring over the entire bandwidth of the frog retinal system is explained in terms of a two-component model.
Abstract: The frog retinal response to light is obtained in the frequency domain. The response is obtained by means of a capillary-type electrode at the cornea. Sinusoidal stimuli are used. The very low frequency range (0.01 to 1 Hz.) is examined. With decreasing frequency, retinal gain increases and the response is more nearly sinusoidal. The increase in sensitivity is thought to result from rod enhancement. The linear-nonlinear-linear transition occurring over the entire bandwidth of the frog retinal system is explained in terms of a two-component model.
TL;DR: These investigations in CF 1 mice were undertaken to establish the usefulness of whole follicle squash preparations in kinetics studies of anagen matrix cell responses to ionizing radiation.
TL;DR: It is concluded that concern with the issue of causing a death remains paramount and Polarization and resolution of these feelings are fundamental and necessary if the demands of society for more abortions are to be met.
TL;DR: Catecholamines stimulate brain hexokinase when Mg 2+ and H + concentration levels are suboptimal and the concentration of the phosphate acceptor ( glucose or 2-deoxyglucose) exceeds the half-saturation level, suggesting a physiologic control mechanism for Hexokinase in which tissue levels of Mg2+, H + and glucose serve as metabolic signals.
TL;DR: It is suggested that the extracellular space estimated by biochemical methods includes both the true interstitial space and the fluid compartment represented by astrocytic cytoplasm, consistent with previously-reported ultrastructural evidence for a high sodium content of corticalastrocytes.
TL;DR: The chances of adverse emotional side reactions to L‐dopa appear to be minimal when the dosage level does not exceed 4 Gm.
Abstract: This paper reports a study, using systematic observation, interview, and psychological examination, in a sample of 34 patients receiving L‐dopa for parkinsonism. Our results do not support the suggestion that disturbances of mood and behavior are to be expected. We concluded that the chances of adverse emotional side reactions to L‐dopa appear to be minimal when the dosage level does not exceed 4 Gm. per day and the subjects are emotionally stable prior to treatment.