The Hertz Corporation

About: The Hertz Corporation is a based out in . It is known for research contribution in the topics: Population & Soil water. The organization has 9562 authors who have published 11044 publications receiving 447929 citations. The organization is also known as: Hertz Rental Car & Hertz Rent-a-Car.

Fluid Shear Stress Modulation of Gene Expression in Endothelial Cells

Abstract: The vascular endothelium, lining the blood vessel wall, is constantly exposed to wall shear stresses generated by flowing blood. Gene regulation, critical for endothelial cell function, depends on complex interactions at the promoter level and utilizes overlapping signal transduction cascades to activate the expression of genes involved in many biological processes.

Application of a generic fast gradient liquid chromatography tandem mass spectrometry method for the analysis of cytochrome P450 probe substrates

Abstract: A liquid chromatography tandem mass spectrometry (LC/MS/MS) method has been developed for the fast routine analysis of selected CYP450 probe substrate metabolites in microsomal incubations, with no sample pretreatment. This has allowed fast and simple assessment of the potential effects which drug candidates may or may not have on the metabolism of specific CYP450 probe substrates, providing information which can then be used to rationalize in vivo interaction studies required in the clinic. This methodology takes advantage of fast gradient chromatography as a generic means of sample separation and analysis. It provides high throughput analysis compared to conventional gradient HPLC, with no significant loss in chromatographic performance. © 1998 John Wiley & Sons, Ltd.

Structural analyses of purified human immunodeficiency virus type 1 intracellular reverse transcription complexes.

Abstract: Retroviruses copy their RNA genome into a DNA molecule, but little is known of the structure of the complex mediating reverse transcription in vivo We used confocal and electron microscopy to study the structure of human immunodeficiency virus type 1 (HIV-1) intracellular reverse transcription complexes (RTCs) Cytoplasmic extracts were prepared 3, 4, and 16 h after acute infection by Dounce homogenization in hypotonic buffer RTCs were purified by velocity sedimentation, followed by density fractionation in linear sucrose gradients and dialysis in a large pore cellulose membrane RTCs had a sedimentation velocity of approximately 350 S and a density of 134 g/ml and were active in an endogenous reverse transcription assay Double labeling of nucleic acids and viral proteins allowed specific visualization of RTCs by confocal microscopy Electron microscopy revealed that RTCs are large nucleoprotein structures of variable shape consisting of packed filaments ca 6 nm thick Integrase and Vpr are associated with discrete regions of the 6-nm filaments The nucleic acids within the RTC are coated by small proteins distinct from nucleocapsid and are partially protected from nuclease digestion

The Velocity and Temperature Dependence of Rubber Friction

Abstract: It is shown experimentally that the velocity of frictional gliding under constant tangential stress of rubber on glass and on silicon carbide cloth is, in the first approximation, an exponential function of the reciprocal absolute temperature and of the tangential stress. It is suggested that frictional gliding of rubber is a rate process.