Institution
Wake Forest Institute for Regenerative Medicine
About: Wake Forest Institute for Regenerative Medicine is a based out in . It is known for research contribution in the topics: Stem cell & Transplantation. The organization has 741 authors who have published 1238 publications receiving 56676 citations.
Topics: Stem cell, Transplantation, Regenerative medicine, Tissue engineering, Mesenchymal stem cell
Papers published on a yearly basis
Papers
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TL;DR: 3D bioprinting is being applied to regenerative medicine to address the need for tissues and organs suitable for transplantation and developing high-throughput 3D-bioprinted tissue models for research, drug discovery and toxicology.
Abstract: Additive manufacturing, otherwise known as three-dimensional (3D) printing, is driving major innovations in many areas, such as engineering, manufacturing, art, education and medicine. Recent advances have enabled 3D printing of biocompatible materials, cells and supporting components into complex 3D functional living tissues. 3D bioprinting is being applied to regenerative medicine to address the need for tissues and organs suitable for transplantation. Compared with non-biological printing, 3D bioprinting involves additional complexities, such as the choice of materials, cell types, growth and differentiation factors, and technical challenges related to the sensitivities of living cells and the construction of tissues. Addressing these complexities requires the integration of technologies from the fields of engineering, biomaterials science, cell biology, physics and medicine. 3D bioprinting has already been used for the generation and transplantation of several tissues, including multilayered skin, bone, vascular grafts, tracheal splints, heart tissue and cartilaginous structures. Other applications include developing high-throughput 3D-bioprinted tissue models for research, drug discovery and toxicology.
4,841 citations
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TL;DR: An integrated tissue–organ printer (ITOP) that can fabricate stable, human-scale tissue constructs of any shape is presented and the incorporation of microchannels into the tissue constructs facilitates diffusion of nutrients to printed cells, thereby overcoming the diffusion limit of 100–200 μm for cell survival in engineered tissues.
Abstract: A challenge for tissue engineering is producing three-dimensional (3D), vascularized cellular constructs of clinically relevant size, shape and structural integrity. We present an integrated tissue-organ printer (ITOP) that can fabricate stable, human-scale tissue constructs of any shape. Mechanical stability is achieved by printing cell-laden hydrogels together with biodegradable polymers in integrated patterns and anchored on sacrificial hydrogels. The correct shape of the tissue construct is achieved by representing clinical imaging data as a computer model of the anatomical defect and translating the model into a program that controls the motions of the printer nozzles, which dispense cells to discrete locations. The incorporation of microchannels into the tissue constructs facilitates diffusion of nutrients to printed cells, thereby overcoming the diffusion limit of 100-200 μm for cell survival in engineered tissues. We demonstrate capabilities of the ITOP by fabricating mandible and calvarial bone, cartilage and skeletal muscle. Future development of the ITOP is being directed to the production of tissues for human applications and to the building of more complex tissues and solid organs.
1,960 citations
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TL;DR: The isolation of human and rodent amniotic fluid–derived stem (AFS) cells that express embryonic and adult stem cell markers are reported and examples of differentiated cells derived from human AFS cells and displaying specialized functions include neuronal lineage cells secreting the neurotransmitter L-glutamate or expressing G-protein-gated inwardly rectifying potassium channels.
Abstract: Stem cells capable of differentiating to multiple lineages may be valuable for therapy. We report the isolation of human and rodent amniotic fluid-derived stem (AFS) cells that express embryonic and adult stem cell markers. Undifferentiated AFS cells expand extensively without feeders, double in 36 h and are not tumorigenic. Lines maintained for over 250 population doublings retained long telomeres and a normal karyotype. AFS cells are broadly multipotent. Clonal human lines verified by retroviral marking were induced to differentiate into cell types representing each embryonic germ layer, including cells of adipogenic, osteogenic, myogenic, endothelial, neuronal and hepatic lineages. Examples of differentiated cells derived from human AFS cells and displaying specialized functions include neuronal lineage cells secreting the neurotransmitter L-glutamate or expressing G-protein-gated inwardly rectifying potassium channels, hepatic lineage cells producing urea, and osteogenic lineage cells forming tissue-engineered bone.
1,843 citations
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TL;DR: Engineered bladder tissues, created with autologous cells seeded on collagen-polyglycolic acid scaffolds, and wrapped in omentum after implantation, can be used in patients who need cystoplasty.
1,706 citations
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TL;DR: Carbon nanotubes may play an integral role as unique biomaterial for creating and monitoring engineered tissue as well as imparting novel properties such as electrical conductivity into the scaffolds may aid in directing cell growth.
995 citations
Authors
Showing all 741 results
Name | H-index | Papers | Citations |
---|---|---|---|
Anthony Atala | 125 | 1235 | 60790 |
Camillo Ricordi | 94 | 845 | 40848 |
Paul Abrams | 91 | 505 | 51539 |
Paolo De Coppi | 88 | 595 | 32332 |
Christopher R. Chapple | 88 | 864 | 29975 |
Karl-Erik Andersson | 87 | 748 | 33703 |
James J. Yoo | 81 | 491 | 27738 |
Shay Soker | 72 | 257 | 24230 |
Paul Gatenholm | 68 | 269 | 16951 |
Maria B. Grant | 68 | 404 | 17546 |
David F. Williams | 67 | 246 | 18737 |
Robert Lanza | 65 | 249 | 21136 |
Ashok K. Hemal | 58 | 441 | 12246 |
George J. Christ | 56 | 257 | 10846 |
Colin E. Bishop | 53 | 143 | 9663 |