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Showing papers in "Archives of Biochemistry and Biophysics in 1951"


Journal ArticleDOI
TL;DR: The evolution of oxygen by illuminated chloroplasts does not involve the intermediate production of H 2 O 2 in a form available to the enzyme catalase, and there is no evidence of a generalized reducing power in plants as part of the photosynthetic mechanism.

805 citations


Journal ArticleDOI
TL;DR: Viscosity measurements and ultracentrifugal experiments show no difference between fibr inogen and fibrinogen incubated with thrombin, both kept at pH 4.85, although the progressing action of thromin can be demonstrated.

436 citations


Journal ArticleDOI
TL;DR: It is concluded that the compound found with treatment of UDPG with an enzyme of S. fragilis is uridine diphosphate galactose, and the bearing of this finding on the mechanism of action of UDPG is discussed.

386 citations


Journal ArticleDOI
TL;DR: Radioactivity measurements on the degradation products of known C 14 -labeled compounds have shown a very low cross-contamination between individual carbon positions.

358 citations



Journal ArticleDOI
TL;DR: The consumption of oxygen by illuminated chloroplasts in the presence of catalase and ethanol reported earlier is shown to be stimulated by prior Hill reaction with quinone, which results in a preparation capable of oxidizing ascorbic acid and glutathione at rates twice that of any other light, reaction of chloroplast.

239 citations


Journal ArticleDOI
TL;DR: In this paper, a systematic study was made of the photochemical action of methylene blue on amino acids, and it was shown that tyrosine, tryptophan, histidine, methionine, and cystine were highly reactive during the photooxidation.

188 citations


Journal ArticleDOI
TL;DR: A soluble enzyme has been isolated from baker's yeast which catalyzes the reduction of pyridine nucleotides by several aldehydes with acetaldehyde, the most rapidly utilized substrate, and the oxidative product is acetic acid.

165 citations



Journal ArticleDOI
TL;DR: Because of the sensitivity and precision of the methods used, the action spectrum for the conversion of protochlorophyll in the leaf is known with an accuracy approaching that of the absorption spectrum of extracted protoch chlorophyll.

146 citations


Journal ArticleDOI
M. Bier1, F.F. Nord1
TL;DR: Calcium and manganese ions are effective protectors of crystallinetrypsin in alkaline solutions and this high isoelectric point is correlated with the amino acid composition of trypsin and its amide nitrogen content.

Journal ArticleDOI
TL;DR: Vitamin A is reversibly dehydrogenated to vitamin A aldehyde (retinene) in isolated retinal rods and in liver extracts containing alcohol dehydrogenase and coenzyme 1, probably involved in the utilization of vitamin A for the regeneration of bleached visual purple.

Journal ArticleDOI
TL;DR: Among a considerable number of yeast species, representing nearly all yeast genera, only six were found which were capable of causing certain changes in pectin, when grown in its presence, the action was found to be due to an exocellular, nonadaptive, polygalacturonase-like enzyme, free of pectinesterase.


Journal ArticleDOI
TL;DR: In this article, the photochemical action of methylene blue on β-lactoglobulin was studied and the first points of attack during photooxidation were the aromatic nuclei of the histidine and tryptophan residues.

Journal ArticleDOI
TL;DR: The results suggest that the cystine and methionine were synthesized in the rumen approximately at an equal rate and were used by the tissues to make new protein in the quantities needed.

Journal ArticleDOI
TL;DR: In this article, a new stepwise degradation procedure for citric, α-ketoglutaric, and glutamic acids has been described, which permits the determination of the isotope concentration of each individual position of the carbon chain of these acids.


Journal ArticleDOI
TL;DR: It was concluded from titrations of native and denatured whole egg white that the ovalbumin contains most, if not all, of the sulfhydryl groups in egg white.



Journal ArticleDOI
TL;DR: It was found that during clotting of fibrinogen larger and larger particles were built up through side-by-side and end-to-end associations of the apparently original fibr inogen particles.

Journal ArticleDOI
TL;DR: A rapid colorimetric method has been described for the quantitative determination of mimosine by using activated carbon as a decolorizing agent and measuring the intensity of Mimosine-ferric chloride color produced.

Journal ArticleDOI
TL;DR: The results indicate that in clots formed without calcium some of the linkages which compose the network are weak associations which can easily rearrange, while inClots formed in the presence of calcium (and serum factor), the network strands are joined by bonds of a far more permanent nature.

Journal ArticleDOI
TL;DR: Adenosine triphosphate, ADP, and ITP are effective maximally in amounts up to 5 × 10 −5 mole and the findings of Straub and Feuer that during polymerization ATP is dephosphorylated to ADP is confirmed.

Journal ArticleDOI
TL;DR: It was concluded that pyridoxine under the conditions of these experiments does not exert a special supplementary action in the production or conservation of polyenoic fatty acids.

Journal ArticleDOI
TL;DR: It has been found that luminescence and the Hill reaction rate, as determined by direct potentiometric measurement, saturate at nearly identical light intensities.



Journal ArticleDOI
TL;DR: It has been shown by the use of acetate labeled with isotopic carbon that the carbon from exogenous acetate is actually incorporated in the rubber formed by guayule.