Showing papers in "Bioelectrochemistry and Bioenergetics in 1991"

TL;DR: A quantitative theory of electroporation of artificial planar lipid bilayer membranes is presented and predicts that the fate in any particular experiment is determined by the properties of the membrane and the duration and amplitude of the charging pulse.

TL;DR: Four major findings relative to transcriptional changes are observed: the increase in mRNA levels is most likely due to an increased rate of transcription (rather than, for example, an increase in RNA stability); frequency-, field strength and time-dependent “windows” are observed relative to quantitative changes in specific transcripts.

TL;DR: In this paper, the authors studied the kinetics of resealing of pore in human red blood cells permeabilized by a single exponential electric field pulse and concluded that depending on the electric pulse parameters there may be three stages of the pore resealing process: (i) rapid decrease in pore size (τr 10 min), (ii) complete resealing, and (iii) rapid restoration of membrane barrier function.

TL;DR: In this article, the authors proposed a method for the detection of organic peroxides based on the electrocatalytic current arising in dependence on the peroxide level in micromolar concentration range.

TL;DR: In this paper, the electrochemistry of cytochrome c at a gold electrode modified with a self-assembled lipid bilayer membrane has been investigated at different ionic strengths, using the electrically neutral lipid egg phosphatidylcholine (PC), either alone or in combination with various amounts of the negatively charged surfactant dihexadecylphosphate (DHP − ).

TL;DR: In this paper, the effect of the phosphate group on the adsorption of the flavins at the TiO 2 surface was investigated and was found to obey the Langmuir isotherm.

TL;DR: The behavior of the esterase/pesticide systems, and the analytical possibilities arising from the inhibiting action exerted by pesticides on esterases were examined and a biosensor for the determination of pesticides did finally result.

TL;DR: The ability of dc fields to inhibit the manifestation of nonlinear dielectric behaviour by these cells is explicable in terms of their ability to act as a field of zero Hz, forcing the excitation out of the amplitude window.

TL;DR: The ways in which the nuclear envelope/membranes can regulate ion fluxes and generate electrical potentials and fields at the nuclear periphery are considered and possible functional consequences of this association are outlined.

TL;DR: The magnitude of the Cole—Cole α of the β-dispersion of yeast cells cannot be ascribed simply to the charging of a static membrane capacitance in cells of differing sizes and/or internal conductivities.

TL;DR: In this paper, the reduction of horse heart cytochrome c has been investigated at a platinum electrode modified with a lipid bilayer membrane (BLM) which immobilized vinyl ferrocene as an electron mediator.

TL;DR: The results suggest that the major source of nonlinear dielectricity within the respiratory chain is either in the branch to cytochrome b 558 or proximal to the site at which HQNO binds, and provides a novel method for the measurement of respiratory activity in situ.

TL;DR: The electrochemical oxidation reactions of α- and β-alanine at a Pt electrode were investigated in aqueous solutions at pH 1, 7, and 13 using steady-state current-potential measurements, cyclic voltammetry, and open circuit potential decay as discussed by the authors.

TL;DR: In this article, stable immobilization of reaction centers from photosynthetic bacterium Rhodobacter sphaeroides R-26 has been achieved at modified platinum electrodes by using reduced cytochrome c and ubiquinone-10.

TL;DR: In this paper, a planar bilayer lipid membrane (BLM) is formed by spreading a solution of phospholipids dissolved in an alkane solvent across an aperture immersed in aqueous solution.

TL;DR: An electrochemical theory of the transport of protons across a biological membrane facilitated by uncouplers of oxidative phosphorylation added to the medium has been developed in this paper, where the interfacial potential differences at the two membrane/solution interfaces are explicitly taken into account.

TL;DR: In this paper, the oxidation current of superoxide anions generated from a single neutrophil stimulated with Immunoglobulin G or phorbol myristate acetate was determined with a carbon fibre microelectrode.

TL;DR: Especially since the 5th Symposium of the Bioelectrochemical Society held at Weimar in 1979, a lot of new electric field effects on membrane bilayers, cells, tissues and animals have been found by several methods, e.g. inductive or capacitative coupling applications of dc or ac field pulses.

TL;DR: In this article, the electron transfer from the modified electrodes to dissolved dihydrolipoamide dehydrogenase (Lip-DH) or viologen accepting pyridine nucleotide oxidoreductase (VAPOR) was tested by NADH formation in potentiostatically controlled electrochemical cells.

TL;DR: In this article, Tsong et al. examined the properties of rectification and active pumping of the ECC model and showed that the rectification effect can be used to explain the ability of a membrane transport system to interact with an electric field.

TL;DR: In line with this trend of synergistic effects between membrane active agents and electrofusion itself, the pulse field strength and width can be lowered remarkably in order to minimize side-effects on cell metabolism and viability.

TL;DR: An improved amino oxidase enzyme electrode has been constructed and applied to the determination of the amount of polyamines present in real samples and the sensor response was stable for more than 32 hours of continuous operation at room temperature and, in the presence of fish or meat homogenates, no change in the signal-to-noise ratio was observed.

TL;DR: In this paper, the interaction of adriamycin (ADR) molecules with human erythrocytes was investigated by radiowave dielectric spectroscopy complemented with electron microscopy methods to elucidate the extent of modification induced by this antitumoural drug in the passive electrical properties of the membrane.

TL;DR: In this article, a voltammetric analysis of the Pb2+ complexation with the DNA shows a cooperative binding of the divalent metal to the negatively charged phosphate residues in the DNA, and the binding isotherm is discussed with reference to various binding models.

TL;DR: In this article, the kinetics of albumin adsorption onto a glassy carbon rotating disk electrode in a phosphate buffer are reported from the time variations of the double-layer capacitance Cd, of the charge transfer resistance Rt and of the Tafel coefficient of the electrochemical reaction b: these three electrical quantities are determined by electrochemical impedance and faradaic current I measurements.

TL;DR: In this paper, a photocell comprising two sheets of the same SnO, electrodes was prepared, and a sharp gradient of light intensity was ensured by using a chromatophore suspension of high optical density to generate an asymmetric distribution of charge.