Showing papers in "Biophysical Chemistry in 1994"

TL;DR: In this article, a simple model was proposed for the thermodynamics of hydration of ions with charges varying from ± 1 to ± 4, depending only on z and their radii r, when taken as spheres.

TL;DR: The importance of compressibility data for characterizing the hydration properties of solutes (particularly, proteins, nucleic acids, and their constituents), while describing how such data can be interpreted to gain insight into role that hydration can play in modulating the stability of and recognition between biologically important compounds is emphasized.

TL;DR: Using Widom's potential distribution theory, a general and a special theorems are derived, by means of which one can judge whether a particular sub-process of an overall process will produce compensating changes in enthalpy and entropy.

TL;DR: Time-resolved fluorescence spectroscopy directly distinguishes between a decrease in fluorescence intensity due to quenching by an excess of unbound Eb from that due to a decreases in Eb binding to double-stranded DNA, suggesting that techniques which measure total steady state fluorescenceintensity of bound Eb in order to infer relative amounts of double- Stranded DNA must be interpreted with caution.

TL;DR: The free energies of cavity formation in water with and without hydrogen bonding potential were computed from a set of Monte Carlo simulation calculations on pure liquid TIP4P water model and on the same model but with the electrostatic charges turned off (Lennard-Jones liquid).

TL;DR: It is shown that the compact native state of a protein is stabilized by the enthalpic interactions between internal groups while the hydration effects of all the groups, except the aliphatic ones, which are exposed upon unfolding destabilize this state.

TL;DR: In this article, the authors compare the dielectric model results with the most current molecular level information to check the basic features of Rashin's calculations, and show that the characteristic structure of contact and solvent-separated minima does not appear in the model results for the pair potentials-of-mean-force for oppositely charged ions in water under physiological thermodynamic conditions.

TL;DR: The results support the validity of applying macroscopic concepts such as interfacial free energy at the molecular level and suggest that molecular volume as well as surface area contributes to the solubility of hydrocarbons in water.

TL;DR: Connectin, also called titin, a giant elastic protein of striated muscle, mainly consists of fibronectin type III and immunoglobulin C2 globular domains, the beta-sheets of which are parallel to the main axis of the molecule.

TL;DR: The proteoglycan aggregate is thus an example for a structure where a polysaccharide forms a template for a supramolecular assembly largely stabilized by protein-protein interactions.

TL;DR: It is shown that the denatured protein scattering profile can be interpreted using a model developed for synthetic polymers in which the chain behaves as a random coil in a good solvent, i.e. with excluded volume interactions.

TL;DR: Two-dimensional NMR in conjunction with energy minimization computation have provided detailed information on the solution structure of single adducts localized in oligonucleotides and Cooperative adduct formation at certain base sequences is suggested by excimer fluorescence.

TL;DR: In this article, the authors review the relationship between convergence and group additivity and show that negative ΔS° arises from changes in internal degrees of freedom and should not be applied to the analysis of protein energetics.

TL;DR: The comparison of hydration of aliphatic and aromatic hydrocarbons leads to the following thermodynamic parameters for these additional interactions between the benzene ring and water at 25 degrees C: enthalpy -5.4 kJ/mol, entropy 26.8 J/K mol and Gibbs energy -13.4 j/mol.

TL;DR: Neutron scattering experiments have been performed on oriented dipalmitoylphosphatidylcholine bilayers containing diphenylhexatriene (DPH) or its trimethylammonium analog (TMA-DPH).

TL;DR: Results suggest that the C-terminus of DP plays a role in the attachment of IF to the desmosome and that a specific site is necessary for interaction with keratin IF.

TL;DR: Hydration and cosolvent interactions of biological macromolecules can be derived from studies of density increments at constant chemical potentials of diffusible solutes through a semipermeable membrane from precision density determinations of solutions dialyzed to equilibrium.

TL;DR: In this article, the initial adsorption of two proteins, ferritin and fibrinogen, from buffer solution onto hydrophilic and hydrophobic silicon surfaces was measured at room temperature with off null ellipsometry in situ at a time resolution of 0.1 second under non-diffusion limited conditions.

TL;DR: In this paper, a second-order curvature elastic energy description for the inverse bicontinuous cubic phases has been developed from existing theories, and it is possible to determine curvature parameters from X-ray diffraction measurements on these phases, and a preliminary analysis of data from a 2:1 (mol: mol) mixture of lauric acid and dilauroylphosphatidylcholine.

TL;DR: It has been found that the fish oil fed rats showed a similarity in their liver phospholipid fatty acid composition like fish liverospholipids, and a drastic fluidization at the C-2 segment of the bilayer, in comparison with their phosphatidylcholines from rat liver, fish liver or shrimps.

TL;DR: The model proposes that the dynamics of ion transport in membrane channels are similar to that in bulk electrolyte solutions, except that ions permeating the pore need to surmount a potential barrier, the height of which can be deduced experimentally.

TL;DR: The Gibbs free energy of adsorption (delta G0ads) was estimated for several amino acids, peptides and proteins in a cation-exchange system suggesting that synergistic binding may play a role for unstructured macromolecules, but not for globular proteins.

TL;DR: Electric evidence is provided for the electrophoretic transport of a highly charged and hydrated macromolecule, probably together with the associated gegen-ions, through the thin hydrophobic film of the lipid bilayer.

TL;DR: The generally observed positive heat capacity change on unfolding can be interpreted as resulting from the difference in work involved in changing the intra- and intermolecular interactions including the weak, highly distance-dependent, van der Waals interactions, for the unfolded and native state, respectively.

TL;DR: The theoretical analysis of the protein denaturation model which includes an irreversible, exothermic and rate-limited step has been improved and applied to the DSC profile of Azurin and it is found that in the case ofAzurin an evidentExothermic effect occurs at the end of the transition.

TL;DR: It is demonstrated that it is necessary to use a precursor Carr-Purcell-Meiboom-Gill pulse train if additional diffusive attenuation is to be avoided in the case of transverse relaxation of fruit and plant stem NMR imaging.

TL;DR: It is found that a simple proportionality to accessible surface with five different atom types allows satisfactory reproduction of hydration enthalpies (T delta S) of over 40 solutes and prediction of experimental dipole moments within 0.1 D is demonstrated.

TL;DR: In this paper, a functional renormalization group method was used to predict two different interaction regimes: (i) a protrusion-dominated regime for sufficiently high temperature, and (ii) a hydration-dominated one for sufficiently low temperature.

TL;DR: It is found that polarization energy contributes up to 37%-61% of the total solute-solvent interaction for the systems considered and is compared with previous theoretical results.

TL;DR: In this paper, the viscous and non-viscous modes of a free bilayer membrane are discussed, taking into account the coupling between bending and the local density of the two monolayers.