Showing papers in "Clinica Chimica Acta in 1969"

TL;DR: A new type of α-amylase starch substrate rendered insoluble by cross-linking and coloured by a dye marker, was shown to be hydrolyzed by α-Amylase from Hyland standard serum, Versatol-E and Enzatrol, and it was possible, with considerable accuracy, to determine α- amylase over more than a thousandfold range of enzyme concentration.

TL;DR: A detailed picture of the phospholipid composition of normal human erythrocytes and plasma was obtained by quantitative two-dimensional thin-layer chromatography on silica gel containing an alkaline component.

TL;DR: Describing seemingly trivial variations in the preparation of blood plasma for amino acid analysis, and in the operation of the automatic amino acid analyzer, that can lead to major errors in the quantitative determination of taurine, phosphoethanolamine, the dicarboxylic amino acids, glutamine, and the disulphide amino acids is described.

TL;DR: A rapid sensitive micromethod is described, for the estimation of serum γ-glutamyltranspeptidase, which is readily adaptable to the performance of batch work.

TL;DR: It is concluded that the absorption of both oxalic acid and calcium may be increased in patients with idiopathic hypercalciuria.

TL;DR: The concentrations of copper, iron, magnesium, and zinc in the hair of eighteen different adult male subjects have been determined by atomic absorption spectroscopy over periods ranging from 4 to 10 months.

TL;DR: Spectrum analysis and thin-layer chromatography proved that there was no difference between the data of authentic 5-HIAA and those of the urine extract, and no interfering compounds could be detected on a thin- layer chromatogram.

TL;DR: Serumα1-antitrypsin, α1-acid glycoprotein, baptoglobin, ceruloplasmin and C-reactive protein are increased, albumin, transferrin, α-and β-lipoprotein are decreased as an early response to tissue trauma and inflammatory disease and in infection these changes are followed by a rise of γ-globulins.

TL;DR: The results demonstrate that the maximal tubular reabsorption capacity can be predicted with a nomogram from measurements of phosphate and creatinine in plasma and in an untimed specimen of urine, from the fasting person.

TL;DR: In this article, the clinical applicability of a new α-amylase substrate, a cross-linked and coloured starch substance, for the determination of α amylase in human urine and serum was shown.

TL;DR: The levels of N -acetyl- β -glucosaminidase, β-galactosidase and β-glucosaidase activity in normal human kidney were determined fluorimetrically as discussed by the authors.

TL;DR: Three methods are described for the determination of ionized calcium in plasma ultrafiltrates and urine involving spectrophotometry, potentiometry and a computer calculation procedure good agreement between all three methods is demonstrated.

TL;DR: A method for the determination of steroid mono- and disulfates in peripheral plasma of a group of young healthy female and male subjects and the specificity was established by mass spectrometric analysis of the effluent from the GLC columns.

TL;DR: A variant alkaline phosphatase has been found in extracts of tumor in 8 of 10 patients with hepatocellular cancer and 2 of 60 control livers.

TL;DR: With this procedure it is possible to determine bile salt concentrations ranging from 10–80 mg/100 ml, and can be extended to include individual bile salts if these are first separated by thin-layer chromatography.

TL;DR: When indium-113m was injected intravenously in ionic form for blood pool scanning, it was bound to plasma transferrin and the excess indium above this level was bound mainly to α2-globulins.

TL;DR: The method has been applied to myelomatosis and cirrhosis and appears capable of separating IgA from IgG and can be applied to other biological fluids including urine and cerebrospinal fluid.

TL;DR: A patient with fat-induced hyperlipemia provides evidence for Pelkonen's hypothesis: namely, vitamin E is first absorbed in the chylomicron fraction and is later transferred to the 3-lipoprotein fraction, where it is almost exclusively found in normal sera in the post-absorptive state.

TL;DR: Methods for the determination of zinc in plasma diluted twenty-fold with 0.1 N HCl, in whole blood diluted one hundred times and urine diluted tenfold, using a Perkin-Elmer 303 atomic absorption spectrophotometer found that Random contamination presents a problem which was best detected by replicate analysis.

TL;DR: Assay of glucocerebrosidase, sphingomyelinase and arylsulfatase A in leucocytes has been diagnostically correlated with Gaucher's disease, Niemann-Pick disease and metachromatic leucodystrophy.

TL;DR: In a controlled study on human volunteers, glutathione reductase activity and urinary excretion of riboflavin were found to be more sensitive than RBC rib oflavin levels to dietary ribofavin.

TL;DR: The general pattern of metabolism of [β-14C]melatonin was found to be similar to that in rats, the major metabolic products being the sulfate and glucuronide conjugates of 6-hydroxymelatonin.

TL;DR: It is suggested that the ratio of the rise in γ-glutamyltranspeptidase to the riseIn alkaline phosphatase is a useful indicator pointing to the site of a lesion in the hepatobiliary system.

TL;DR: Present methods for the assay of GOT and GPT are discussed in the light of recent literature, and it is possible to standardise the solution of NADH against a stable iron standard without the need for an ultraviolet spectrophotometer.

TL;DR: The concentration of low density lipoproteins is an important factor determining the serum level of α-tocopherol, according to estimates performed on the sera of normal subjects and hospital in-patients manifesting a wide variety of clinical conditions.

TL;DR: A remarkable finding in these two patients was the replacement of urinary pseudouridine by an equivalent amount of uracil during separate periods of several consecutive days.

TL;DR: The method is reproducible and an accurate measurement of added calcium phosphate and calcium oxalate crystals can be obtained and the importance of the technique as a method to study renal stone disease is discussed.

TL;DR: The urinary excretion of δ-aminolevulinic acid was from 10 to 250 mg per g of creatinine, i.e., up to a 100-fold increase above the normal range, and the excrete of porphobilinogen and porphyrins fell within the normal ranges or was only slightly elevated.

TL;DR: A sensitive and accurate method for measuring calcium and magnesium in serum and urine by atomic absorption spectrophotometry is described, and possible interference from urinary constituents, flame conditions, the use of a variable atomiser, effect of dilution and other parameters have been investigated.

TL;DR: Previous gas-liquid Chromatographie studies on urinary phenolic and indolic metabolites and accompanying extraction and preparative procedures have been extended and conditions have been devised to minimise O -methylation during the preparation of methyl ester derivatives.