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Journal ArticleDOI

Technical pitfalls leading to errors in the quantitation of plasma amino acids.

Thomas L. Perry, +1 more
- 01 Jul 1969 - 
- Vol. 25, Iss: 1, pp 53-58
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TLDR
Describing seemingly trivial variations in the preparation of blood plasma for amino acid analysis, and in the operation of the automatic amino acid analyzer, that can lead to major errors in the quantitative determination of taurine, phosphoethanolamine, the dicarboxylic amino acids, glutamine, and the disulphide amino acids is described.
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This article is published in Clinica Chimica Acta.The article was published on 1969-07-01. It has received 255 citations till now. The article focuses on the topics: Amino acid & Tryptophan.

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Citations
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Journal ArticleDOI

The pathogenesis of coronary artery disease. A possible role for methionine metabolism.

TL;DR: A reduced ability to metabolise homocysteine in some patients with premature coronary artery disease when this pathway is stressed is suggested.
Journal ArticleDOI

Almost all about citrulline in mammals.

TL;DR: One of the most promising research directions deals with the administration of citrulline as a more efficient alternative to arginine, especially against underlying splanchnic sequestration of amino acids.
Journal ArticleDOI

Retinal degeneration associated with taurine deficiency in the cat.

TL;DR: A sulfur amino acid deficit in the casein diet combined with specific amino acid requirements of the cat appear related to this unique expression of taurine deficiency in cats.
Journal ArticleDOI

Homocysteinemia due to folate deficiency

TL;DR: Observations support the hypothesis that depletion of tissue folate causes homocysteinemia in nonhomocystinuric subjects and relatively normal levels of serum total homocyst(e)ine were observed in four pregnant women with low serum folate, supporting previous suggestions of an influence of female sex hormone(s) in homocysteine metabolism.
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Folic acid--an innocuous means to reduce plasma homocysteine

TL;DR: It is proposed that folic acid in excess acts by enhancing the remethylation of homocysteine to methionine, which confirms a previous report and might be of use as prophylaxis.
References
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Journal ArticleDOI

The free amino acids of human blood plasma.

TL;DR: It has been possible to identify with a high degree of probability twenty-eight ninhydrin-positive substances in protein-free plasma and to determine most of them quantitatively.
Journal ArticleDOI

Ion exchange chromatography of the free amino acids in the plasma of the newborn infant.

TL;DR: It was concluded that heel puncture plasma would be satisfactory for routine clinical purposes and a number of small peaks which have not been noted previously in plasma have been indicated on the chromatogram.
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What are the different aspects of measurement of amino acids in plasma using HPLC?

The resulting improved accuracy in measurement of plasma amino acids may be essential for meaningful studies of some metabolic or nutritional disorders where differences from normal are subtle.