Showing papers in "Cryobiology in 1969"

TL;DR: In this article, the authors found that the optimal cooling velocity when 0.1-ml quantities of cell suspension were rapidly thawed was closer to 100°C per min than to 1°Cper min, and only 25% of rapidly cooled cells survived freezing to −196°C without any additive.

TL;DR: A new freeze-etching module, which permits easy preparation of 4 simultaneous replicas and more than 40 replicas per day, has been developed, and satisfactory specimens of yeast cells, barteria, algae, nematodes, mouse brain, parasitized red blood cells, and virus-infected tobacco cells have been prepared.

TL;DR: Improved results will require the development of a perfusion fluid which permits the capillary membrane to retain its normal permeability characteristics, and which prevents a rise in vascular resistance.

TL;DR: Freeze-etching, as a method of preparing specimens for electron microscopy, is perhaps the best method for visualizing the fine structure of freezing damage to cells.

TL;DR: In this paper, the authors developed a freezing method in which skin is pretreated with 20 to 30% glycerol at 4°C for 1 to 2 hours before freezing.

TL;DR: For example, it is generally agreed that considerable palliation of distressing symptoms caused by incurable cancer may be achieved by cryosurgery, even when other methods have failed or cannot be used as discussed by the authors.

TL;DR: To investigate the effects of cryophylactie agents and subzero cooling in rat hearts, an isolated perfusion system was modified from that originally described by Langendorff, and more than 100 hearts from 400-g albino Sprague Dawley rats were perfused at 37°C.

TL;DR: Cardiac cells treated with a cryoprotectant can survive freezing to liquid nitrogen temperatures, andUltramicroscopic evidence obtained from mammalian liver and smooth muscle cells indicates that their incubation in a balanced salt solution is not entirely innocuous.

TL;DR: Fine structure of these highly vacuolated, high moisture cells typically comprising most plant tissues was extensively altered by all freeze-thaw combinations tried, and Membranous components of the protoplasm were especially affected.

TL;DR: In this article, a technique for the in vivo freezing of whole organs at the operating table has been described using intraarterial helium in conjunction with ambient nitrogen as the cryogenic agents.

TL;DR: Results obtained extend earlier results on phase relations and pH changes in buffer-salt solutions during freezing and are useful in understanding pH and composition changes in the unfrozen phase of frozen biological materials.

TL;DR: Stems of four plants representing a range of plant types were extracted and analyzed for peroxidase isozyme components, indicating that plant peroxide metabolism is strongly influenced by the environment in which the plants are grown.

TL;DR: Glycerol had a greater effect than dimethyl sulfoside because glycerol reduced salt solubility less while it maintained about the same amount of water unfrozen, reducing its effectiveness as an additive.

TL;DR: By comparison with a variety of other electron transport components, the succinate cytochrome c reductase complex was found to be particularly senstive to, and suitable for evaluating, freeze-thaw damage.

TL;DR: Bull spermatozoa in several buffered egg yolk media and in skim milk were exposed at 5°C to varying levels of glycerol for 6 hrs, 30 min, and approximately 10 see prior to freezing, suggesting that the conditions provided by the brief exposure to Glycerol more fully protected the spermatoozoa than did prolonged exposure.

TL;DR: A preliminary study of the suspension of animation incorporating a modified model of Neely's indicates that survival may be possible in a reasonable number of experimental animals after a period of 60 min of asanguineous perfusion.

TL;DR: Differences in subcooling were apparent among unhardened seedlings of citrus varieties, but differences were not a satisfactory index of varietal differences in cold hardiness ratings.

TL;DR: Cryobiotic potentialities on earth, investigating life forms physiological response to temperature, cryotolerance mechanisms, etc.

TL;DR: A method of analysis is described which evaluates the biochemical and physiological status of kidneys after preservation experiments and identifies the injury of preservation in a numerical way and will be helpful in the solution of the problems in at least two of three major areas involved in the successful freeze preservation of whole organs.

TL;DR: Recovery of viable ram sperm was significantly higher for samples frozen at the standard rate than at 1°C per min, and the freezing point, at least in egg yolkcitrate, was recorded before the two rates had differentiated, therefore, the differences in survival due to the freezing rates were produced after the samples had frozen.

TL;DR: Turnip yellow mosaic virus (TYMV) is a simple, icosahedral, RNA-containing plant virus, which is structurally severely injured by in vitro freezing of its water solutions.

TL;DR: Localization of C14-DMSO activity sites along cell membranes of various tissues and organs is interpreted in terms of relative impermeability of cell membranes to pharmacological doses of DMSO solvated in body fluids.

TL;DR: Isolated frog sciatic nerves with or without intact perineural sheaths were exposed to 5, 10, or 15% dimethyl sulfoxide (DMSO) or glycerol in Ringer's solution for 15 min and frozen at −10°C in the presence or absence of these protective agents to distinguished from freeze-thaw damage.

TL;DR: In this article, the viscosity of whole blood taken from rats cooled to a given temperature and that of rats with a body temperature of 35°C and then cooled (the blood) to that given temperature were measured in British Standards Institute U-tube capillary viscometers.

TL;DR: The in vivo survival of rapidly frozen and thawed homologous rabbit platelet concentrates with and without protective agents, was determined by labeling the platelets with Na253Cro4 and ten per cent glycerol as an additive was significantly les effective than DMAC-dextrose and appeared less effective than those specimens in which no additives were used.

TL;DR: Cardiac storage at this time is possible for a few days in experimental studies but only a few hours in the clinical setting, and freezing, the method of greatest potential, has been the most resistant to successful experimental study.

TL;DR: The phenomenon of freeze-thaw-induced latent injury, or deleterious effects of freezing and thawing manifested with time after thawed, was demonstrated by slices of renal cortex of the mouse by correlative evidence for coincident accentuated ultrastructural damage to mitochondria.