Journal of Genetics and Breeding 

About: Journal of Genetics and Breeding is an academic journal. The journal publishes majorly in the area(s): Population & Gene. It has an ISSN identifier of 0394-9257. Over the lifetime, 818 publications have been published receiving 4904 citations.

Genotypic effects on tissue culture response of lettuce cotyledons

Abstract: A wide range of lettuce (Lactuca sativa L.) cultivars representing the four major morphological types have been screened for tissue culture aptitude, in order to identify genotypes especially amenable for genetic manipulation at the cellular and molecular level. The growth response of lettuce cotyledons showed marked genotypic differences on both callus initiation medium and subsequent transfer to a plant regeneration medium. A new regeneration medium consisting of SH salts and vitamins, 30 g/l sucrose, 7 g/l agar, 0.1 mg/l IAA, 0.5 mg/l kinetin and 0.05 mg/l zeatin, at pH 5.8 substantially improved callus induction and shoot regeneration on cotyledons in most cultivars. Lettuce genotypes exhibiting good regeneration include Greenfields and Summer Gem (Crisphead types), Bronze Mignonette and Green Mignonette (Butterhead types), Salad Bowl (Leaf Type) and Cos and Green Cos (Romaine types)

Proposal for combining the classification systems of alleles of Gli-1 and Glu-3 loci in bread wheat (Triticum aestivum L.)

Abstract: A new system of nomenclature for the alleles of the genes encoding some gliadin and low molecular weight glutenin (LMW-GS) in bread wheat (Triticum aestivum L.) is suggested. Gliadins encoded by Gli-A1, Gli-B1 and Gli-D1 and LMW-GS encoded by Glu-A3, Glu-B3 and Glu-D3 are described using the following format; Gli-A1 x, Glu-A3 y, were x is the Gli-1 allele designated by Metakovsky (1991) and y is the Glu-3 allele designated by Gupta and Shepherd (1990). The short hand version of the nomenclature is to use only the gliadin allele, e.g. Gli-B1b. However, if there can be more than one Glu-3 allele associated with a particular Gli-1 allele, or vice versa, both alleles should be written, e.g. Gli-A1f, Glu-A3a, Gli-B1m, Glu-B3i or Gli-D1b, Glu-D3c. In this pubblication we have also used the format Gli-A1, Glu-A3 x; y. A list of 18 cultivars is given to cover variation found in the set of 49 cultivars studied. The seed of this definite set is available on request

Intron-mediated improvement of a selectable marker gene for plant transformation using Agrobacterium tumefaciens

Abstract: In binary vectors, the antibiotic resistance gene used for selection of transformed plant cells is also usually expressed in the transforming Agrobacterium cells. This expression gives the bacterium antibiotic resistance, an unnecessary advantage on selective medium containing the antibiotic. Insertion of a castor bean catalase-1 (CAT-1) gene intron or a Parasponia andersonii haemoglobin gene intron into the coding region of the selectable marker gene, hph, completely abolished the expression of the gene in Agrobacterium, rendering it susceptible to hygromycin B. Use of these modified binary vectors minimized the overgrowth of Agrobacterium during plant transformation. Both of the introns were correctly spliced in plant cells and significantly enhanced hph gene expression in transformed rice tissue. The presence of these introns in the hph coding sequence not only maintained the selection efficiency of the hph gene, but with the CAT-1 intron also substantially increased the frequency of rice transformation. Transgenic lines with an intron-hph gene generally contained fewer gene copies and produced substantially more mRNA of the predicted size. Our results also indicate that transgenic plants with many copies of the transgene were more likely to show gene silencing than plants with 1-3 copies.