Showing papers in "Journal of Virological Methods in 1988"

TL;DR: A rapid, sensitive and automated assay procedure was developed for the in vitro evaluation of anti-HIV agents, which significantly reduced labor time as compared to the trypan blue exclusion method, and permits the evaluation of large numbers of compounds for their anti-hIV activity.

TL;DR: Because in vitro co-infection of CD4 cells by HBLV and HIV leads to enhanced degeneration, this raises the possibility that infection in AIDS patients by both viruses can aggravate the HIV-induced immunodeficiency.

TL;DR: 500 sera representing healthy blood donors and a random representation of the U.S. population collected 10 years ago were screened by ELISA for antibody reactivity with purified, disrupted HBLV virions, finding no antibody cross-reactivity between H BLV and other human herpesviruses were detected.

TL;DR: In vitro enzymatic amplification was applied to detect hepatitis B virus DNA sequences in serum using the polymerase chain reaction (PCR) to amplify a 128 bp DNA fragment including a 112 nucleotide long sequence complementary to a region in the S gene of the HBV genome.

TL;DR: In situ hybridization with biotinylated DNA probes is as sensitive as techniques using 35S-labeled probes for detecting HPV infections in routine cervical biopsies or smears.

TL;DR: Clinical and serologic studies suggest an association of CFS with all of the human herpesviruses, particularly Epstein-Barr virus (EBV) and the recently-discovered human B-lymphotropic virus (HBLV) or human herpesvirus-6; neither EBV nor HBLV has yet been shown to play a causal role in the illness.

TL;DR: Serologic studies showed in situ hybridization on lymph node biopsies was positive for HHV-6 genome in 1 out of 5 sarcoidosis lymph nodes.

TL;DR: The ELISA for B19 antigen was shown to be as sensitive as DNA hybridisation and immunoblotting might be used in pregnancy to determine the time of maternal infection.

TL;DR: PCR was used to recognize human B-lymphotropic virus (HBLV or HHV-6) specific sequences in various tumors as well as in the blood of patients with AIDS and in situ hybridization of clinical specimens using radiolabelled RNA probes or hapten-labelled DNA probes was used.

TL;DR: Large scale purification of enveloped HHV-6 virions and the viral DNA was achieved by continuous flow centrifugation utilizing sucrose gradients, DNAse 1 treatment and banding on 10-30% Dextran T10 gradients.

TL;DR: Antibody titers were generally higher in patients with simultaneous EBV or CMV infection, yet crossreactivity appeared essentially no problem, and HHV-6 thus is ubiquitous like other herpesviruses.

TL;DR: The gI-ELISA provides a basis for ADV-eradication programmes, which introduces a novel concept in the control of animal virus diseases.

TL;DR: The immunoblot studies reveal an enormous diversity in EBV-specific polypeptides recognised by different individual patients, both for IgM and IgG.

TL;DR: Investigation of the appearance and development of serum IgG and IgM against human CMV structural polypeptides in sequential sera from renal transplant recipients during the first 1-3 months of primary CMV infection showed that the first IgG to appear is specific for aPolypeptide of 66 kDa and these antibodies appear either alone or together with others against another polypePTide of 82 kDa.

TL;DR: A recombinant virus Ac373-HB was detected and purified from the transfection mixture of wild type and recombinantirus in the baculovirus expression system using a combination of limiting dilution and DNA dot-blot hybridization.

TL;DR: It appears, that HBLV, similar to other herpesviruses such as Epstein-Barr virus, usually causes a silent seroconversion, yet may be associated with variable clinical pathology when persisting in an active state.

TL;DR: Twelve different potyviruses and cytoplasmic inclusion proteins were purified from a range of plant species utilizing a single purification protocol, with results suitable for the production of antisera with high specific titers and low titers against healthy plant antigens.

TL;DR: The results show that antibodies from different individual donors may recognise EBV-MCP through binding to a variety of different epitopes, demonstrating the potential of using systematic peptide synthesis to define serologically relevant linear epitopes on large and relatively unexplored viral polypeptides.

TL;DR: It appears that some herpes viruses, during their initial encounter with susceptible cells, can be forced into preferential productive infection by the process of centrifugation, but after a lapse of an hour or more, centrifugations can no longer do this and most of the infected cells fail to replicate virus.

TL;DR: Delta is defined as the distance of the mean OD ratio of the sample population from the cut-off measured in standard deviation units, and the derivation of this measurement and its usefulness when evaluating ELISA tests is discussed.

TL;DR: An antigen capture indirect enzyme linked immunosorbent assay (ELISA) was developed to detect influenza nucleoprotein antigen in nasal secretions from horses infected with A/equine/H3N8 viruses.

TL;DR: HSB-2 cell cultures productively infected with human herpesvirus-6 were treated with the antiviral drugs phosphonoformic acid (PFA), acyclovir (ACV), and gancyclovIR (DHPG), and ACV and DHPG showed significant toxic effects on uninfected HSB-2 cells, yet only incompletely inhibited viral expression upon infection of the cells.

TL;DR: The assay exhibits comparable sensitivity to two other methods used for such measurements, is relatively rapid, may be adapted for screening large numbers of samples and involves minimal handling of infectious virus.

TL;DR: With this system, rabies-virus antigen was detected in neurons, glandular acinar cells, and vascular endothelial cells more effectively than by immunofluorescence, especially when tissues were enzyme-digested with pronase before immunoperoxidase staining.

TL;DR: It is hoped that new techniques such as polymerase chain reaction will provide new insights into these questions by allowing detection of viral DNA from tissue biopsies obtained early in the course of disease and by providing a method to identify viral isolates that do not grow well in vitro.

TL;DR: Pretreatment of sera or plasma of HIV carriers by heating at 70 degrees C for 10 min at an acidic pH enabled us to estimate antigens efficiently in immune complexes more precisely.

TL;DR: IgM antibodies against ECHO 3 antigen as determined by SPRIST were found to be cross-reactive over a broad range of enterovirus types and positive results were recorded with sera from infections with Coxsackie A9, B3, B4, B5, Echo 3, 9, 17, 18, 25 and 30.

TL;DR: It is suggested that centrifugation enhances MCMV infection by three mechanisms related to reversibility of binding by increasing the rate of virus association, increasing the length of time each virus particle is bound, and increasing the probability of virus being taken into the cell.

TL;DR: A micromethod for the detection of human immunodeficiency virus (HIV) and other retroviruses in cell culture supernatants is described which applies a DEAE ion exchanger for recovery of polynucleotides synthesized in vitro by the retroviral reverse transcriptase.

TL;DR: The combined data from conventional estimates of sensitivity and specificity and delta values suggests that the Abbott assay is the test of choice for screening purposes.