Journal ArticleDOI
A method for staining epoxy sections for light microscopy.
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TLDR
A technique for staining sections of osmium-fixed, epoxy-embedded tissues for light microscopy using aqueous toluidine blue at pH 11.1 and does not require prior removal of embedding medium, which permits better utilization of the full resolving power of the light microscope.About:
This article is published in Journal of Ultrastructure Research.The article was published on 1961-08-01. It has received 800 citations till now. The article focuses on the topics: Oil immersion & Electron microscope.read more
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Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
TL;DR: Six stages of oocyte development in the anuran Xenopus laevis can be divided into six stages based on the anatomy of the developing oocyte, and these stages have been correlated with physiological and biochemical data related to oogenesis.
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STUDIES ON INFLAMMATION: I. The Effect of Histamine and Serotonin on Vascular Permeability: An Electron Microscopic Study
Guido Majno,G. E. Palade +1 more
TL;DR: The mechanism, whereby histamine and serotonin increase the permeability of blood vessels, was studied in the rat by means of the electron microscope, and electron microscopic findings suggested that the endothelial cells become partially disconnected along the intercellular junctions.
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Functions of coated vesicles during protein absorption in the rat vas deferens
TL;DR: Large coated vesicles serve as heterophagosomes to transport absorbed protein to lysosomes, and some small coated vedicle serve as primary lysOSome to transport hydrolytic enzymes from the Golgi complex to multivesicular bodies.
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Science and Art in Preparing Tissues Embedded in Plastic for Light Microscopy, with Special Reference to Glycol Methacrylate, Glass Knives and Simple Stains
TL;DR: When compared with paraffin, GMA offers opportunities for simpler, quicker procedures and yields sections of superior quality, greater information content, and less distortion.
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The fine structure of the gastric mucosa in the bat.
Susumu Ito,Robert J. Winchester +1 more
TL;DR: The fine structure of the various cell types in this species is fundamentally similar to that of the corresponding cell types of other mammals, but the relative cell numbers and distribution are somewhat different.
References
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Journal ArticleDOI
Improvements in epoxy resin embedding methods.
TL;DR: Epoxy embedding methods of Glauert and Kushida have been modified so as to yield rapid, reproducible, and convenientembedding methods for electron microscopy.
Journal ArticleDOI
Embedding in epoxy resins for ultrathin sectioning in electron microscopy.
TL;DR: More rapid than previous techniques, this method gives blocks which do not fracture unduly on trimming and provides sections of soft tissues at 1 μ for phase contrast microscopy, as well as ultrathin sections which cut as easily with glass knives as sections of methacrylate.
Journal ArticleDOI
s-Collidine as a Basis for Buffering Fixatives
H. Stanley Bennett,John H. Luft +1 more
TL;DR: The search for a medium which might offer improvements from the point of view of stability and buffering capacity for Palade's buffered osmic fixative was focused on s-collidine, which has a pK of about 7.4.
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A Rapid Method for Localization of Tissue Structures or Lesions for Electron Microscopy
TL;DR: This method enables rapid localization of desired lesions whilc sectioning and ensures that the lesion is in that portion of the block trimmed for thin sections, and allows determination of the depth and adequacy of the zone of optimal fixation in the original block.