Journal ArticleDOI
A micromanipulation system with dynamic force-feedback for automatic batch microinjection
TLDR
The experimental results demonstrate that the technique of position control with dynamic penetration-force feedback is practicable for automatic batch microinjection applications.Abstract:
In this paper, we report the development of a prototype micromanipulation system for automatic batch microinjection of zebrafish embryos. Such automatic batch processing is made possible by (i) the development of a machine vision algorithm to identify the number of embryos in a batch and to locate the centerline of each embryo, (ii) the integration of a piezoresistive micro-force sensor with a micropipette to measure the penetration force of the embryo in real time and (iii) the synthesis of a position control with dynamic force feedback by exploiting the characteristics of the force profile associated with the microinjection process. The effectiveness of this prototype micromanipulation system has been demonstrated in an experiment. The experimental results demonstrate that the technique of position control with dynamic penetration-force feedback is practicable for automatic batch microinjection applications.read more
Citations
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Journal ArticleDOI
FluidFM: Combining Atomic Force Microscopy and Nanofluidics in a Universal Liquid Delivery System for Single Cell Applications and Beyond
André Meister,Michael Gabi,Pascal Behr,Philipp Studer,Janos Vörös,Philippe Niedermann,Joanna Bitterli,Jérôme Polesel-Maris,Martha Liley,Harry Heinzelmann,Tomaso Zambelli +10 more
TL;DR: The universality and versatility of the fluidFM will stimulate original experiments at the submicrometer scale not only in biology but also in physics, chemistry, and material science.
Journal ArticleDOI
Robotic Cell Injection System With Position and Force Control: Toward Automatic Batch Biomanipulation
TL;DR: A robotic cell-injection system for automatic injection of batch-suspended cells is proposed and experimental results demonstrate the effectiveness of the proposed approach.
Journal ArticleDOI
A Force Control Approach to a Robot-assisted Cell Microinjection System
TL;DR: A force-control-based cell injection approach that is capable of regulating the penetration force in a desired force trajectory is developed and experiments performed on fish embryos verify the effectiveness of the proposed approach.
Journal ArticleDOI
Microfluidics-enabled phenotyping, imaging, and screening of multicellular organisms
TL;DR: This paper reviews the technologies that have been invented in the last few years on high-throughput phenotyping, imaging, screening, and related techniques using microfluidics and highlights other applications that can be developed in the future.
Journal ArticleDOI
Nanonewton Force Sensing and Control in Microrobotic Cell Manipulation
TL;DR: The experimental results demonstrate that the microgripper and the control system are capable of rapid contact detection and reliable force-controlled micrograsping to accommodate variations in size and stiffness of cells with a high degree of reproducibility.
References
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Journal ArticleDOI
Intracytoplasmic sperm injection in the mouse.
TL;DR: Intracytoplasmic sperm injection was successful in the mouse when a piezo-driven micropipette was used instead of a mechanically driven conventional pipette, and approximately 70% of sperm-injected oocytes developed into blastocysts in vitro.
Journal ArticleDOI
Zebrafish genetics and vertebrate heart formation
TL;DR: Recent work shows that a newly initiated genetic approach in zebrafish is already making significant contributions to understanding the development of the vertebrate heart, an organ that contains several vertebrate-specific features.
Journal ArticleDOI
Biological Cell Injection Using an Autonomous MicroRobotic System
Yu Sun,Bradley J. Nelson +1 more
TL;DR: A microrobotic system is developed that is capable of performing automatic embryo pronuclei DNA injection autonomously through a hybrid visual servoing control scheme and the experimental result shows that the injection success rate is 100%.
Journal ArticleDOI
Mechanical property characterization of mouse zona pellucida
TL;DR: In this article, a microelectromechanical system-based multiaxis cellular force sensor has been used to conduct mouse ZP force sensing, establishing a quantitative relationship between applied forces and biomembrane structural deformations on both mouse oocytes and embryos.
Journal ArticleDOI
Cloned zebrafish by nuclear transfer from long-term-cultured cells
TL;DR: It is reported that fertile transgenic zebrafish can be obtained by nuclear transfer using embryonic fibroblast cells from long-term cultures, demonstrating that slowly dividing nuclei from cultured cells can be reprogrammed to support rapid embryonic development and sets up a foundation for targeted genetic manipulation in zebra fish.