A Model for Predicting Ionic Equilibrium Concentrations in Cell Walls
Hervé Sentenac,Claude Grignon +1 more
TLDR
A model which describes the ionic atmosphere in the cell walls as a result of simultaneous electrostatic interactions between free ions (Donnan effect) and specific association equilibria, including acido-basic ones correctly accounted for the whole set of experimental results and predicted the values of the unmeasurable local concentrations and pH.Abstract:
Purified cell walls were prepared from roots of Horse bean (Vicia faba L., var. minor) and Yellow Lupine (Lupinus luteus L.). Two methods were used: (a) grinding and (b) lysis of the endocellular contents by Triton X-100. The Ca2+, Mg2+, and K+ contents were determined after incubation in various solutions in such a manner that the measurements gave access to the undisturbed equilibrium contents. The results were used to test a model which describes the ionic atmosphere in the cell walls as a result of simultaneous electrostatic interactions between free ions (Donnan effect) and specific association equilibria, including acido-basic ones. This model correctly accounted for the whole set of experimental results and predicted the values of the unmeasurable local concentrations and pH.read more
Citations
More filters
Journal ArticleDOI
Handbook of Chemistry and Physics
TL;DR: While the book is a standard fixture in most chemical and physical laboratories, including those in medical centers, it is not as frequently seen in the laboratories of physician's offices (those either in solo or group practice), and I believe that the Handbook can be useful in those laboratories.
Journal ArticleDOI
The Acid Growth Theory of auxin-induced cell elongation is alive and well.
David L. Rayle,R E Cleland +1 more
TL;DR: The Acid Growth Theory, which states that when exposed to auxin, susceptible cells excrete protons into the wall (apoplast) at an enhanced rate, resulting in a decrease in apoplastic pH, activates wall-loosening processes, the precise nature of which is unknown.
Journal ArticleDOI
Structure and properties of pectin gels in plant cell walls
TL;DR: The structural characterization of pectins and the gels which they form, in relation to auxin-induced extension growth, the ripening of fruit, and cellular recognition are discussed in this article.
Journal ArticleDOI
pH and Ionic Conditions in the Apoplast
C. Grignon,H. Sentenac +1 more
TL;DR: The Apoplast as an Ion Exchanger as mentioned in this paper was used as a diffusion barrier in the early days of the Apollo project, and it has been used as an ion reservoir and an ion filter in the last few decades.
Journal ArticleDOI
Calcium and the cell wall
TL;DR: It appears that the interactions between calcium ions and cell walls play a key role in plant physiology and calcium can significantly affect this factor, the density of non-diffusible charges.
References
More filters
Journal ArticleDOI
A modified uronic acid carbazole reaction
T. Bitter,Helen Muir +1 more
TL;DR: It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.
Journal ArticleDOI
Handbook of Chemistry and Physics
TL;DR: While the book is a standard fixture in most chemical and physical laboratories, including those in medical centers, it is not as frequently seen in the laboratories of physician's offices (those either in solo or group practice), and I believe that the Handbook can be useful in those laboratories.
Journal ArticleDOI
Ionic Relations of Cells of Chara Australis II. The Indiffusible Anions of the Cell Wall
J Dainty,AB Hope,Christine Denby +2 more
TL;DR: In this paper, isolated cell walls from Ohara australi8 have been extended to measure the concentration of the endogenous anions of the wall and the pK of the acids which ionize to give these anions.
Journal ArticleDOI
The influence of surface charge on the kinetics of ion-translocation across biological membranes
TL;DR: Rate equations have been developed which describe the concentration dependence for ion-translocation across charged membranes for those cases in which the translocation process can be considered to be formally equivalent with an enzymic process of a Michaelis-Menten type and it is shown that even when an ion does not bind to the translocated sites the Km is affected by increasing concentrations of this ion, a phenomenon which is not expected when the membrane is not charged.
Journal ArticleDOI
Exchange Properties of Isolated Cell Walls of Lemna minor L.
TL;DR: The rational selectivity coefficients of the carboxylic groups of the walls during exchanges of divalent ions against monovalent ones are estimated, showing a purely physicochemical mechanism of "regulation" of the exchanges in the cells walls of Lemna minor L.