Journal ArticleDOI
A modified uronic acid carbazole reaction
T. Bitter,Helen Muir +1 more
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TLDR
It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.About:
This article is published in Analytical Biochemistry.The article was published on 1962-10-01. It has received 5798 citations till now. The article focuses on the topics: Uronic acid & Carbazole.read more
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New method for quantitative determination of uronic acids
TL;DR: A new method for determination of uronic acids with meta-hydroxydiphenyl is introduced, which is simpler, quicker, more sensitive, and more specific than other methods, and it needs lesser amounts of fluid.
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Autoimmunity to type II collagen an experimental model of arthritis.
TL;DR: Since homologous type II collagen emulsified in oil without bacterial preparations regularly causes the disease, this new animal model of arthritis represents a unique example of experimentally-inducible autoimmunity to a tissue component.
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Fluorometric assay of DNA in cartilage explants using Hoechst 33258.
TL;DR: A simple two-step fluorometric assay of DNA in cartilage explants, utilizing the bisbenzimidazole dye Hoechst 33258, is described, which offers advantages over other established DNA assays of Cartilage and may be especially useful in metabolic studies of cartilageExplants.
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A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage cultures.
TL;DR: A rapid spectrophotometric procedure is described for the estimation of sulfated glycosaminoglycans in cartilage cultures that is substantially free from interference, is sensitive to less than 1 microgram (4 micrograms/ml) of chondroitin sulfate, and provides a simple alternative to the traditional methods for gly cosaminoglycan determinations.
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Measurement of uronic acids without interference from neutral sugars
TL;DR: It is found that a small amount of sulfamate suppresses color production by a 20-fold excess of some neutral sugars without substantial sacrifice of the sensitive detection of uronic acids by the diphenyl reagent.
References
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Studies in immunochemistry. 11. The action of dilute alkali on the N-acetylhexosamines and the specific blood-group mucoids.
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