A plaque assay for the simian rotavirus SAII.
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A sensitive, quantitative and reproducible plaque assay for the measurement of the simian rotavirus SAII is described, finding that MA-104 cells were the most sensitive.Abstract:
A sensitive, quantitative and reproducible plaque assay for the measurement of the simian rotavirus SAII is described. Plaque formation required the presence of the facilitators pancreatin or trypsin and diethylaminoethyl-dextran in the agar overlay. SAII produced plaques in three continuous primate cell lines: MA-104, CV-1 and LLC-MK2. MA-104 cells were the most sensitive.read more
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References
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Rotavirus isolation and cultivation in the presence of trypsin.
TL;DR: This procedure resulted in virus isolation from five of five samples that contained virus particles, as demonstrated by electron microscopy, and four of seven samples where virus particles could not be observed but were considered positive by the presence of immunofluorescent-staining cells in feces.
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Journal ArticleDOI
Enzyme replacement therapy of exocrine pancreatic insufficiency in man. Relations between in vitro enzyme activities and in vivo potency in commercial pancreatic extracts.
TL;DR: Lipase activity in vitro correlated with potency in vivo for tablet and capsules, with tablets and capsules being effective in reducing steatorrhea by 56.1±9 per cent and 48.6±10 per cent.
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Plaque assay of neonatal calf diarrhea virus and the neutralizing antibody in human sera.
S Matsuno,Sakae Inouye,R Kono +2 more
TL;DR: It was demonstrated that some human sera contained neutralizing antibody to this agent, and titration of neutralizing antibodies to neonatal calf diarrhea virus was made feasible.
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