Journal ArticleDOI
A rapid and easy method for the detection of microbial cellulases on agar plates using gram's iodine
Reads0
Chats0
TLDR
This is the first report on the use of Gram’s iodine for the detection of cellulase production by microorganisms using plate assay, which is rapid and efficient and can be easily performed for screening large numbers of microbial cultures of both bacteria and fungi.Abstract:
Screening for cellulase-producing microorganisms is routinely done on carboxymethylcellulose (CMC) plates. The culture plates are flooded either with 1% hexadecyltrimethyl ammonium bromide or with 0.1% Congo red followed by 1 M NaCl. In both cases, it takes a minimum of 30 to 40 minutes to obtain the zone of hydrolysis after flooding, and the hydrolyzed area is not sharply discernible. An improved method is reported herein for the detection of extracellular cellulase production by microorganisms by way of plate assay. In this method, CMC plates were flooded with Gram’s iodine instead of the reagents just mentioned. Gram’s iodine formed a bluish-black complex with cellulose but not with hydrolyzed cellulose, giving a sharp and distinct zone around the cellulase-producing microbial colonies within 3 to 5 minutes. The new method is rapid and efficient; therefore, it can be easily performed for screening large numbers of microbial cultures of both bacteria and fungi. This is the first report on the use of Gram’s iodine for the detection of cellulase production by microorganisms using plate assay.read more
Citations
More filters
Journal ArticleDOI
Isolation, Characterization, and Metabolic Profiling of Ceratorhiza hydrophila from the Aquatic Plant Myriophyllum spicatum
TL;DR: The enzyme analyses showed its ability to produce protease and cellulase enzymes, and the ethyl acetate extract of C. hydrophila showed intermediate antibacterial activity against Streptococcus pneumonia, Micrococcus luteus, and Staphylococcus aureus.
Journal ArticleDOI
Evaluating the Effect of Rumen Microorganisms in the Codigestion Process of Swine Manure and Rice Husk
Journal ArticleDOI
Isolation and Characterization of Plant Growth Promoting Rhizospheric Bacteria From Limonium stocksii
TL;DR: The present investigation was carried out to isolate and characterize the PGPR from the rhizospheric region of Limonium stocksii, a halophyte, and six isolates showed PGP traits as well as produced extracellular hydrolytic enzymes.
Journal ArticleDOI
Isolation and optimization of the fermentation condition of cellulolytic microbial isolates from cassava waste water
TL;DR: Cassava processing water regarded as waste water could be an alternative source of microorganisms capable of producing cellulase enzyme for industrial purposes.
References
More filters
Journal ArticleDOI
Cellulases and related enzymes in biotechnology
TL;DR: The present article is an overview of the biotechnological state-of-the-art for cellulases and related enzymes.
Journal ArticleDOI
The biological degradation of cellulose
Pierre Béguin,Jean-Paul Aubert +1 more
TL;DR: The study of cellulolytic enzymes at the molecular level has revealed some of the features that contribute to their activity and an increasing number of three-dimensional structures are becoming available for cellulases and xylanases belonging to different families, which will provide paradigms for molecular modeling of related enzymes.
Journal ArticleDOI
The Properties of Fungal and Bacterial Cellulases with Comment on their Production and Application
TL;DR: Structure de la cellulose, sources et production d'enzymes cellulolytiques: regulation, secretion, mutation, mutation et clonage, multiplicite des enzymes.