Journal ArticleDOI
A rapid and easy method for the detection of microbial cellulases on agar plates using gram's iodine
Reads0
Chats0
TLDR
This is the first report on the use of Gram’s iodine for the detection of cellulase production by microorganisms using plate assay, which is rapid and efficient and can be easily performed for screening large numbers of microbial cultures of both bacteria and fungi.Abstract:
Screening for cellulase-producing microorganisms is routinely done on carboxymethylcellulose (CMC) plates. The culture plates are flooded either with 1% hexadecyltrimethyl ammonium bromide or with 0.1% Congo red followed by 1 M NaCl. In both cases, it takes a minimum of 30 to 40 minutes to obtain the zone of hydrolysis after flooding, and the hydrolyzed area is not sharply discernible. An improved method is reported herein for the detection of extracellular cellulase production by microorganisms by way of plate assay. In this method, CMC plates were flooded with Gram’s iodine instead of the reagents just mentioned. Gram’s iodine formed a bluish-black complex with cellulose but not with hydrolyzed cellulose, giving a sharp and distinct zone around the cellulase-producing microbial colonies within 3 to 5 minutes. The new method is rapid and efficient; therefore, it can be easily performed for screening large numbers of microbial cultures of both bacteria and fungi. This is the first report on the use of Gram’s iodine for the detection of cellulase production by microorganisms using plate assay.read more
Citations
More filters
Journal ArticleDOI
Co-culturing of Novel Bacillus Species Isolated from Municipal Sludge and Gut of Red Wiggler Worm for Improving CMCase Activity
TL;DR: In this paper, the carboxymethyl cellulose (CMC) agar plate showed a larger zone of clearance with Gram's iodine staining, and the 16S rRNA gene sequences of the strains EF2 and OW1-1 were identified as a gram-positive Bacillus sp.
Journal ArticleDOI
Isolation and identification of a native microbial consortium for the coffee pulp degradation above 2000 masl
Marcela Nancy Arteaga Cuba,Josue Otoniel Dilas-Jiménez,Norberto Villanueva Díaz,Ofelia Carol Cernaqué Miranda,Segundo Medardo Tafur Santillan,José Olivera García,Carlos Mugruza-Vassallo +6 more
TL;DR: Different bacterial and fungi consortium were found supporting other recent work done with one type of consortium, that would contribute to the recovery and conservation of the coffee ecosystem.
Journal ArticleDOI
Isolation and Selection of Streptomyces Species from Semi-arid Agricultural Soils and Their Potential as Producers of Xylanases and Cellulases.
Y.E. Castañeda-Cisneros,Yuridia Mercado-Flores,Miguel Angel Anducho-Reyes,Jorge Álvarez-Cervantes,B Ponce-Lira,Z Evangelista-Martínez,Alejandro Téllez-Jurado +6 more
TL;DR: Streptomyces species were isolated from agricultural soils of the Mezquital Valley of Mexico and the production of endoglucanases (CMCase) and xylanases (Xyl) in Solid-State Cultivation (SSC) was evaluated, finding that the greatest enzymatic activity was observed in particle size 12.
Patent
Endophyte compositions and methods for improvement of plant traits
TL;DR: In this paper, the authors describe methods and materials for modulating the characteristics of a plant, having been heterologously disposed to an endophyte or a plurality of endophytes, or derived from a plant reproductive element.
Proceedings ArticleDOI
Isolation, identification and cellulase-producing condition optimization of Bacillus subtilis Q3 strain
TL;DR: In this article, a cellulase-producing bacterium, designated Q-3, was isolated from silage corn on CMC-Na Congo Red medium plates and further identified as Bacillus subtilis using morphological investigation and 16S rDNA sequencing analysis.
References
More filters
Journal ArticleDOI
Cellulases and related enzymes in biotechnology
TL;DR: The present article is an overview of the biotechnological state-of-the-art for cellulases and related enzymes.
Journal ArticleDOI
The biological degradation of cellulose
Pierre Béguin,Jean-Paul Aubert +1 more
TL;DR: The study of cellulolytic enzymes at the molecular level has revealed some of the features that contribute to their activity and an increasing number of three-dimensional structures are becoming available for cellulases and xylanases belonging to different families, which will provide paradigms for molecular modeling of related enzymes.
Journal ArticleDOI
The Properties of Fungal and Bacterial Cellulases with Comment on their Production and Application
TL;DR: Structure de la cellulose, sources et production d'enzymes cellulolytiques: regulation, secretion, mutation, mutation et clonage, multiplicite des enzymes.