Journal ArticleDOI
A rapid and easy method for the detection of microbial cellulases on agar plates using gram's iodine
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TLDR
This is the first report on the use of Gram’s iodine for the detection of cellulase production by microorganisms using plate assay, which is rapid and efficient and can be easily performed for screening large numbers of microbial cultures of both bacteria and fungi.Abstract:
Screening for cellulase-producing microorganisms is routinely done on carboxymethylcellulose (CMC) plates. The culture plates are flooded either with 1% hexadecyltrimethyl ammonium bromide or with 0.1% Congo red followed by 1 M NaCl. In both cases, it takes a minimum of 30 to 40 minutes to obtain the zone of hydrolysis after flooding, and the hydrolyzed area is not sharply discernible. An improved method is reported herein for the detection of extracellular cellulase production by microorganisms by way of plate assay. In this method, CMC plates were flooded with Gram’s iodine instead of the reagents just mentioned. Gram’s iodine formed a bluish-black complex with cellulose but not with hydrolyzed cellulose, giving a sharp and distinct zone around the cellulase-producing microbial colonies within 3 to 5 minutes. The new method is rapid and efficient; therefore, it can be easily performed for screening large numbers of microbial cultures of both bacteria and fungi. This is the first report on the use of Gram’s iodine for the detection of cellulase production by microorganisms using plate assay.read more
Citations
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Characterization of Target Organisms from Environmental Origin for Rapid Bioremediation of Hexamine
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Pseudomonas schmalbachii sp. nov., isolated from the gut of a millipede (Trigoniulus corallinus) from a coconut tree.
Matan Shelomi,Wen-Ming Chen,Hsin-Kuang Chen,Hsin-Ying Lee,Chiu-Chung Young,Shih-Yao Lin,Shwu-Jen Liaw +6 more
TL;DR: In this article, a Pseudomonas isolate, Milli4T, was cultured from the digestive tract of the common Asian millipede, Trigoniulus corallinus, and whole genome sequencing suggested that this isolate represents a new species with average nucleotide identity (OrthoANIu) values of around 83.9-87.7% with its closest relatives.
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Isolation of alkali-thermotolerant cellulolytic bacteria from spent mushroom substrate
Posted ContentDOI
Fungi with history: Unveiling the mycobiota of historic documents of Costa Rica
Efraín Escudero-Leyva,Sofía Vieto,Roberto Avendaño,Diego Rojas-Gätjens,P.R. Agüero,Carlos Pacheco,Mavis L. Montero,Priscila Chaverri,Max Chavarría +8 more
TL;DR: The physicochemical characteristics and mycobiota of five key historic documents from Costa Rica, including the Independence Act of Costa Rica from 1821, are studied, suggesting that cotton-based paper is the most resistant to microbial colonization.
References
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Journal ArticleDOI
Cellulases and related enzymes in biotechnology
TL;DR: The present article is an overview of the biotechnological state-of-the-art for cellulases and related enzymes.
Journal ArticleDOI
The biological degradation of cellulose
Pierre Béguin,Jean-Paul Aubert +1 more
TL;DR: The study of cellulolytic enzymes at the molecular level has revealed some of the features that contribute to their activity and an increasing number of three-dimensional structures are becoming available for cellulases and xylanases belonging to different families, which will provide paradigms for molecular modeling of related enzymes.
Journal ArticleDOI
The Properties of Fungal and Bacterial Cellulases with Comment on their Production and Application
TL;DR: Structure de la cellulose, sources et production d'enzymes cellulolytiques: regulation, secretion, mutation, mutation et clonage, multiplicite des enzymes.