A simple and rapid DNA microextraction method for plant, animal, and insect suitable for RAPD and other PCR analyses.
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This article is published in Genome Research.The article was published on 1993-08-01 and is currently open access. It has received 179 citations till now. The article focuses on the topics: RAPD.read more
Citations
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Journal ArticleDOI
Advances in molecular marker techniques and their applications in plant sciences
TL;DR: A new class of advanced marker techniques has emerged, primarily derived from combination of earlier basic techniques, thereby revealing genetic variation through increased genome coverage.
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Seed dispersal by animals: exact identification of source trees with endocarp DNA microsatellites
José A. Godoy,Pedro Jordano +1 more
TL;DR: In this paper, the authors used the multilocus genotype at simple sequence repeat loci of the woody endocarp, a tissue of maternal origin, to identify the source tree for 82.1% of the seeds collected in seed traps and hypothesize that the remaining 17.9% of sampled seeds come from other populations.
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Arabidopsis Trehalose-6-Phosphate Synthase 1 Is Essential for Normal Vegetative Growth and Transition to Flowering
TL;DR: The arrested tps1/tps1 embryonic state could be rescued using a dexamethasone-inducible AtTPS1 expression system enabling generation of homozygous mutant plants.
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A new DNA extraction method for high‐throughput marker analysis in a large‐genome species such as Triticum aestivum
TL;DR: A new flexible method for plant DNA extraction that allows a high-throughput of samples in a short time without the need for freezing or lyophilizing the plant material is presented.
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Mechanical transmission of lumpy skin disease virus by Aedes aegypti (Diptera: Culicidae).
TL;DR: Mosquitoes that had fed upon lesions of LSDV-infected cattle were able to transmit virus to susceptible cattle over a period of 2–6 days post-infective feeding, suggesting that mosquito species are competent vectors.
References
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A simple and rapid method for the preparation of plant genomic DNA for PCR analysis.
K Edwards,C Johnstone,C Thompson +2 more
TL;DR: This work has developed a method for the rapid extraction of small amounts of plant genomic DNA suitable for PCR analysis that is applicable to a variety of plant species and has the added advantage of not requiring any phenol or chloroform extraction.
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A rapid and inexpensive method for isolation of total DNA from dehydrated plant tissue
Thomas H. Tai,Steven D. Tanksley +1 more
TL;DR: The advantages of the described procedure are that it is fast, does not require expensive equipment (e.g., lyophilizer) and can be used in situations where large numbers of samples must be extracted.
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RAPD and other PCR-based analyses of plant genomes using DNA extracted from small leaf disks.
J M Deragon,B S Landry +1 more
TL;DR: A nondestructive, early DNA diagnostic system to implement marker-assisted selection in plant breeding programs and fast DNA polymorphism analyses based on site-specific or arbitrary DNA amplification has been developed.
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Rapid isolation of rice and maize DNA for analysis by random-primer PCR
James H. Oard,Sridhar Dronavalli +1 more
TL;DR: PCR was used recently to generate and map genetic markers from controlled sexual crosses that were linked to disease resistance genes and requires DNA to be isolated from several near-isogenic, backcross or segregating individuals in a population.