Journal ArticleDOI
A Solid-Phase Protein Assay: Quantitation of Protein in the Nanogram Range
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TLDR
A solid-phase protein assay for the determination of protein concentrations in the nanogram range is described and used in combination with an antigen-specific ELISA it gives a reliable ratio of specific versus total protein.About:
This article is published in Analytical Biochemistry.The article was published on 1993-06-01. It has received 3 citations till now. The article focuses on the topics: Lowry protein assay & Bradford protein assay.read more
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Journal ArticleDOI
Use of an extracorporeal circuit to evaluate effects of intraluminal distention and decompression on the equine jejunum.
Jorge E. Nieto,Linda M. Van Hoogmoed,Sharon J. Spier,Nicholas J. Vatistas,Jack R. Snyder,Brenna L. Timmerman +5 more
TL;DR: Intraluminal distention of the jejunum followed by decompression increased mucosal permeability and injury and decreased responses to prokinetic agents.
REVIEW Colorimetric protein assay techniques
TL;DR: It is suggested that the most critical issue in the use of a chromogenic protein assay for the characterization of a biopharmaceutical is the selection of a standard for the calibration of the assay; it is crucial that the standard be representative of the sample.
Journal ArticleDOI
A procedure for the detection of free thiol-containing proteins on a polyvinylidene difluoride membrane.
TL;DR: Results suggested that sulfhydryl-containing proteins were specifically and semi-quantitatively identified by membrane biotinylation with Biotin-BMCC.