Journal ArticleDOI
A study of the phosphorylated intermediate of sarcoplasmic reticulum atpase
G. Inesi,G. Inesi,E. Maring,E. Maring,Alexander J. Murphy,Alexander J. Murphy,B.H. McFarland,B.H. McFarland +7 more
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TLDR
A mechanism is proposed for SR ATPase, in which Ca 2+ binding to the membrane occurs as a consequence of site modification induced by ATP binding, and activates phosphoryl transfer and formation of a phosphorylated intermediate.About:
This article is published in Archives of Biochemistry and Biophysics.The article was published on 1970-05-01. It has received 148 citations till now. The article focuses on the topics: Hydroxylamine & ATPase.read more
Citations
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Membrane formation by the adenosine triphosphatase of sarcoplasmic reticulum.
TL;DR: It is concluded that the purified ATPase enzyme is a functional subunit and is likely to be a structural subunit of the sarcoplasmic reticulum membrane.
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Phosphorylation of the sarcoplasmic reticulum membrane by orthophosphate. Inhibition by calcium ions
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Role of water, hydrogen ion, and temperature on the synthesis of adenosine triphosphate by the sarcoplasmic reticulum adenosine triphosphatase in the absence of a calcium ion gradient
de Meis L,Martins Ob,Alves Ew +2 more
References
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Journal ArticleDOI
Phosphorus Assay in Column Chromatography
TL;DR: If the highest accuracy was not required, the following manipulations simplified and speeded multiple total phosphorus determinations on the eluates from column chromatographic separations.
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Adenosine triphosphate-linked concentration of calcium ions in a particulate fraction of rabbit muscle
Setsuro Ebashi,Fritz Lipmann +1 more
TL;DR: ATPase and ATP-dependent calcium ion concentration was studied with a membrane fraction isolated from homogenized rabbit skeletal muscle by differential centrifugation, indicating that it consists mainly of resealed tubules and vesicles of the endoplasmic reticulum.
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A phosphorylated intermediate in adenosine triphosphate-dependent sodium and potassium transport across kidney membranes.
TL;DR: On the basis of tracer exchange experiments with (Na+ + K+)-ATPase in crab nerve, Skou proposed an enzyme-high energy phosphate inter- mediate, E - P, consistent with reciprocal competitive inhibitory effects.
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Reaction Mechanism of the Ca++-dependent ATPase of Sarcoplasmic Reticulum from Skeletal Muscle I. Kinetic Studies
Taibi Yamamoto,Yuji Tonomura +1 more
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The phosphorylation of the membranal protein of the sarcoplasmic vesicles during active calcium transport.
TL;DR: The activity of the calcium pump is not affected appreciably when the vesicles are treated with hydrazine under optimal conditions for phosphorylation, and the stability of the isolated phosphoprotein has its optimum at pH 2.5.