Actinomycin D-binding in vivo: active chromatin preferred.
TLDR
Escherichia coliRNA polymerase and the endogenous engaged RNA polymerase I were used as specific probes to monitor the physiologically inactive and active nucleolar chromatin template function, respectively.About:
This article is published in FEBS Letters.The article was published on 1983-05-30 and is currently open access. It has received 17 citations till now. The article focuses on the topics: Nucleolar chromatin & Chromatin.read more
Citations
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Journal ArticleDOI
Structural and sequence-dependent aspects of drug intercalation into nucleic acids.
TL;DR: Information gained from X-ray crystallographic studies on drug-nucleic acid complexes is described, with emphasis on the intercalation process, and relevant data from NMR experiments are examined.
Journal ArticleDOI
Psoralen-crosslinking of DNA as a probe for the structure of active nucleolar chromatin☆
TL;DR: It is concluded that the extent of psoralen-crosslinking in chromatin DNA is diagnostic for the structure of undistorted chromatin, as well as for the chromatin in the non-transcribed spacer of the ribosomal DNA.
Journal ArticleDOI
Nup50 is required for cell differentiation and exhibits transcription-dependent dynamics.
TL;DR: Nup50 is a mobile nucleoporin with a pronounced presence both at the nuclear pore complex and in the nucleoplasm that can move between these different localizations, dependent on active transcription by RNA polymerase II.
Journal ArticleDOI
Reversible and irreversible changes in nucleosome structure along the c-fos and c-myc oncogenes following inhibition of transcription.
TL;DR: It is suggested that the intercalation of actinomycin D into the DNA of active nucleosomes can lock the transcription complex into an "unfolded" but potentially active configuration.
Journal Article
Actinomycin D and its mechanisms of action
Marcin Koba,Jerzy Konopa +1 more
TL;DR: In the literature not enough convincing evidence has been proposed that could indicate one particular mechanism of action as responsible for the biological activity of actinomycin D, so the slow dissociation of acting as an anticancer drug from DNA complexes, its photodynamic activity and free radical formation, as well as other biochemical effects of activity of this drug are reviewed.
References
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Journal ArticleDOI
A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter
TL;DR: A modification of the naphthalene-dioxane-PPO liquid scintillator has been described which will allow up to 3.0 ml of an aqueous solution to be counted as mentioned in this paper.
Journal ArticleDOI
Chromosomal subunits in active genes have an altered conformation
Harold Weintraub,Mark Groudine +1 more
TL;DR: The results indicate that active genes are probably associated with histones in a subunit conformation in which the associated DNA is particularly sensitive to digestion by deoxyribonuclease I.
Book ChapterDOI
[105] Determination of DNA concentration with diphenylamine
TL;DR: The chapter focuses on the later modifications for the determination of DNA especially in microorganisms and animal tissues and presents the modifications described by Burton, Croft and Lubran, and Giles and Myers.
Journal ArticleDOI
Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei.
Annie Garel,Richard Axel +1 more
TL;DR: Although histones reside on the transcriptionally active ovalbumin genes in the oviduct, the organization of proteins about this gene renders it highly sensitive to deoxyribonuclease I, which confirms the observations of H. Weintraub and M. Groudine and suggests an aspect of structure that may be necessary to permit transcription of the chromatin complex.
Book ChapterDOI
Actinomycin and Nucleic Acid Function
E. Reich,Irving H. Goldberg +1 more
TL;DR: A model recently proposed for the reaction of actinomycin with DNA and to the implications of this model for the template function of helical nucleic acids for the role of RNA metabolism in many systems is related particularly to this discussion.
Related Papers (5)
Studies of the binding of actinomycin and related compounds to DNA
Werner Müller,Donald M. Crothers +1 more