Journal ArticleDOI
Aequorin luminescence: relation of light emission to calcium concentration--a calcium-independent component.
TLDR
Light emission from the calcium-sensitive bioluminescent protein aequorin was measured at calcium ion concentrations of 10(-9) to 10(-2) molar and the complete relation is well described by a two-state model involving three calcium-binding sites.Abstract:
Light emission from the calcium-sensitive bioluminescent protein aequorin was measured at calcium ion concentrations of 10(-9) to 10(-2) molar. At very low Ca2+ concentations, light emission is independent of calcium ion concentration. The maximum slope of the log-log plot of light as a function of calcium ion concentration is about 2.5. The complete relation is well described by a two-state model involving three calcium-binding sites.read more
Citations
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Effects of pH on the myofilaments and the sarcoplasmic reticulum of skinned cells from cardiace and skeletal muscles.
Fabiato A,F Fabiato +1 more
TL;DR: It was concluded that the effects of acidosis on the cardiac sarcoplasmic reticulum accentuate the depressive action of decreasing pH on the myofilaments, which may explain the pronounced depression of contractility observed during acidosis in cardiac muscle.
Journal ArticleDOI
Measurement of Intracellular Calcium
TL;DR: A number of methods have been developed to measure intracellular Ca2+.
Journal ArticleDOI
Measurement of Ca2+ concentrations in living cells
Journal ArticleDOI
A free calcium wave traverses the activating egg of the medaka, Oryzias latipes.
TL;DR: It is concluded that the fertilization wave in the medaka egg is propagated by calcium-stimulated calcium release, primarily from some internal sources other than the large cortical vesicles.
Journal ArticleDOI
Fluorescence and bioluminescence measurement of cytoplasmic free calcium.
Peter H. Cobbold,T J Rink +1 more
TL;DR: The technique has been rapidly developed with the invention of the superior dyes, fura-2 and indo1, and technologies for monitoring [Ca2], in single cells, whole fields of identified cells, and even localized areas within cells, with improvements in time resolution down to the millisecond range.
References
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Journal ArticleDOI
Extraction, Purification and Properties of Aequorin, a Bioluminescent Protein from the Luminous Hydromedusan, Aequorea
Journal ArticleDOI
Depolarization and calcium entry in squid giant axons.
Baker Pf,Hodgkin Al,Ridgway Eb +2 more
TL;DR: The conclusion is that the rise in calcium concentration resulting from stimulation is confined to the peripheral part of the axon and that the diffusion coefficient of aequorin in axoplasm is about 4 × 10−7 cm2/sec.
Journal ArticleDOI
On the relationships between membrane potential, calcium transient and tension in single barnacle muscle fibres
C. C. Ashley,E. B. Ridgway +1 more
TL;DR: The calcium‐sensitive photoprotein aequorin has been used to follow the rapid changes in intracellular calcium concentration that occur during the contraction of single muscle fibres from the barnacle Balanus nubilus, Darwin.
Journal ArticleDOI
Permeability of cell junction depends on local cytoplasmic calcium activity
TL;DR: It will be shown that uncoupling ensues when the rise in [Ca2+]i occurs at the junction, but not when it occurs at other regions in the cell, as well as when the concentration of free ionised calcium in cytoplasm increases.
Journal ArticleDOI
Komplexone XXIX. Ein grosser Chelateffekt besonderer Art
TL;DR: In this article, the stability constants of the 1:1 complexes of Ca2+, Cd2+ and Hg2+ have been determined for nearly the whole series of these complexons and a few values have been obtained also for the complexes of Mg2+, Sr2+, Ba2+, and Go2+.