Journal ArticleDOI
Alkaline protease in the larvae of the army worm, Spodoptera litura
TLDR
The alkaline protease activity in the gut of Spodoptera litura was found to increase with the development of larvae and decreased with the onset of pupation, and the preparation was not completely homogeneous and showed three clearly separable protein bands by polyacrylamide gel electrophoresis.About:
This article is published in Insect Biochemistry.The article was published on 1976-01-01. It has received 33 citations till now. The article focuses on the topics: Protease & Spodoptera litura.read more
Citations
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Journal ArticleDOI
Midgut protease activities in 12 phytophagous lepidopteran larvae: Dietary and protease inhibitor interactions
TL;DR: Larval midgut extracts from 12 lepidopteran species from 5 families were surveyed for protease activities, finding that nine species appear to have a digestive system based largely on trypsin and elastase activities while three had lower levels of these two enzymes and increased levels of chymotrypsin activity.
Journal ArticleDOI
Examination of midgut luminal proteinase activities in six economically important insects
TL;DR: Feeding studies found no mortality nor stunting associated with feeding high levels of trypsin inhibitors to boll weevil or tobacco budworm larvae despite the fact that these proteins were very good inhibitors of midgut proteolytic activity in vitro.
Journal ArticleDOI
Luminal proteinases from Plodia interpunctella and the hydrolysis of Bacillus thuringiensis CryIA(c) protoxin.
Brenda Oppert,Karl J. Kramer,Karl J. Kramer,Donovan E. Johnson,Steve J. Upton,W. H. McGaughey +5 more
TL;DR: Several serine proteinase inhibitors reduced gut enzyme activities, with two soybean trypsin inhibitors, two potato inhibitors, and chymostatin the most effective in preventing protoxin hydrolysis.
Journal ArticleDOI
Purification and characterization of three alkaline proteases from the gut of the larva of army worm, Spodoptera litura
TL;DR: Three alkaline proteases from the larval gut of Spodoptera litura have been purified by gel filtration on Sephadex G-75 and ion exchange chromatography on DEAE-SephadeX A-50 and inhibited by phenylmethylsulphonyl fluoride suggesting the presence of serine in the active site.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
Journal ArticleDOI
Crystalline soybean trypsin inhibitor : ii. general properties.
TL;DR: Crystalline soy protein when denatured is readily digestible by pepsin, and less readily by chymotrypsin and by trypsin, which results in a proportional gain in the inhibiting activity.
Journal ArticleDOI
Spectrophotometric studies; the crystallographic and optical properties of the hemoglobin of man in comparison with those of other species.
Journal ArticleDOI
Crystalline soybean trypsin inhibitor
TL;DR: Crystalline soy protein when denatured is readily digestible by pepsin, and less readily by chymotrypsin and by trypsin, which results in a proportional gain in the inhibiting activity.
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