Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds.
Franky R. G. Terras,Hilde M. E. Schoofs,M. F. C. De Bolle,F. Van Leuven,S B Rees,Jozef Vanderleyden,Bruno P. A. Cammue,Willem F. Broekaert +7 more
TLDR
The radish 2S storage albumins were identified as the second novel class of antifungal proteins that inhibit growth of different plant pathogenic fungi and some bacteria, however, their antimicrobial activities are strongly antagonized by cations.About:
This article is published in Journal of Biological Chemistry.The article was published on 1992-08-05 and is currently open access. It has received 582 citations till now. The article focuses on the topics: Raphanus & Plant defensin.read more
Citations
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Peptide Antimicrobial Agents
TL;DR: The structural requirements of peptides for antiviral and antibacterial activities are evaluated in light of the diverse set of primary and secondary structures described for host defense peptides.
Journal ArticleDOI
The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins
L.C. van Loon,E.A. Van Strien +1 more
TL;DR: SAR, likewise, is a generally occurring phenomenon, that engenders an enhancement of the defensive capacity of plants in response to necrotizing infections, associated with the development of systemic acquired resistance (SAR) against further infection by fungi, bacteria and viruses.
Journal ArticleDOI
Plant defensins: novel antimicrobial peptides as components of the host defense system.
TL;DR: A novel class of plant peptides whose structural and functional properties resemble those of insect and mammalian defensins are characterized, which are one class among the numerous types of Cys-rich antimicrobial peptides.
Journal ArticleDOI
The male determinant of self-incompatibility in Brassica.
TL;DR: Loss- of-function and gain-of-function studies prove that the SCR gene product is necessary and sufficient for determining pollen self-incompatibility specificity, possibly by acting as a ligand for the stigmatic receptor.
Journal ArticleDOI
Small cysteine-rich antifungal proteins from radish: their role in host defense.
Franky R. G. Terras,Kristel Eggermont,Kovaleva,Natasha V. Raikhel,Rupert W. Osborn,Kester A,Rees Sb,Sophie Torrekens,Van Leuven F,Jozef Vanderleyden +9 more
TL;DR: It is demonstrated that two homologous, 5-kD cysteine-rich proteins designated Raphanus sativus-antifungal protein 1 and Rs-AFP2 are located in the cell wall and occur predominantly in the outer cell layers lining different seed organs, and are preferentially released during seed germination after disruption of the seed coat.
References
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PatentDOI
Measurement of protein using bicinchoninic acid
TL;DR: This new method maintains the high sensitivity and low protein-to-protein variation associated with the Lowry technique and demonstrates a greater tolerance of the bicinchoninate reagent toward such commonly encountered interferences as nonionic detergents and simple buffer salts.
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Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining
TL;DR: Comparison of isoelectric focusing and SDS‐containing gels and a simple spot test on thin gellayers show that the detection sensitivity depends not so much on the type of proteins but rather on their structure.
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The Mineral Nutrition of Higher Plants
David T. Clarkson,John B. Hanson +1 more
TL;DR: This review of mineral nutrients shall not be considering two most important, but frequently reviewed, aspects of the subject, namely biological fixation of N/sub 2/ and its assimilation and mechanisms of membrane transport.
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Calcium and plant development
Peter K. Hepler,Randy Wayne +1 more
TL;DR: The Ca2+ -MEDIATED PROCESS ES means that the aim of the project is to improve the quality of life of people living in the Mediterranean region by developing a sustainable and resilient infrastructure.
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Biochemical and molecular characterization of three barley seed proteins with antifungal properties.
TL;DR: Three proteins from barley (Hordeum vulgare L.) seeds which synergistically inhibit the growth of fungi measured in a microtiter well assay are purified and their biological roles and potential use in genetic engineering studies are discussed.