Journal ArticleDOI
Androgenic response from cultured anthers of a leguminous tree,Cassia siamea Lam.
TLDR
Microscopic examination of the anthers cultured at the late uninucleate or early bi-celled stages, after 7–14 days of culture, revealed many multicellular structures at various stages of development, thus indicating the pollen origin of callus.Abstract:
Anthers ofCassia siamea, on culture on B5 medium supplemented with coconut milk (15%, v/v), 2,4-D (2 mg/l), and kinetin (0.5 mg/l), split open after one to two weeks of inoculation and eject a callus mass. Microscopic examination of the anthers cultured at the late uninucleate or early bi-celled stages, after 7–14 days of culture, revealed many multicellular structures at various stages of development, thus indicating the pollen origin of callus. Callus cells also showed the haploid chromosome number (n=14).read more
Citations
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Book ChapterDOI
Published doubled haploid protocols in plant species
TL;DR: These protocols provide procedures for production of DH in such major crops as wheat, maize, barley, rapeseed or potato and also in other crop species where this technology is more advanced, or in other words, where a relatively high frequency of doubled haploids was obtained.
Journal ArticleDOI
Toward doubled haploid production in the Fabaceae: Progress, constraints, and opportunities
Janine Croser,Monika M. Lulsdorf,P.A. Davies,Heather Clarke,K.L. Bayliss,Nalini Mallikarjuna,Kadambot H. M. Siddique +6 more
TL;DR: A review of the literature has enabled us to identify some general similarities between the protocols developed for haploid plant induction across the various legumes, which will assist researchers to identify approaches suited to their target Fabaceae species.
Journal ArticleDOI
In vitro androgenesis in tree species: an update and prospect for further research.
TL;DR: This review article intends to bring together in one place all the important variables used by different workers, that influence androgenic potential immensely like, stage of anther or microspore at culture, media composition, combinations and concentrations of growth regulators, and additives, and be a worthy guide to all the prospective workers in this area and in designing their experiments further.
References
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Journal ArticleDOI
Nutrient requirements of suspension cultures of soybean root cells.
TL;DR: The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2, 4- d) has been designed.
Journal ArticleDOI
Cell Division and Differentiation of Embryos in the Pollen Grains of Datura in vitro
Sipra Guha,Satish C. Maheshwari +1 more
TL;DR: Evidence is reported which shows that the mature anthers of Datura produce numerous embryo-like structures when cultured on Nitsch's medium containing 15 per cent coconut milk originate from the pollen grains.
Journal ArticleDOI
Haploids from pollen grains - retrospect and prospect
TL;DR: The culture of isolated pollen is seen as a promising line of further research as it has the potential of offering several advantages over the culture of whole anthers, particularly for producing pure lines for crop improvement.
Journal ArticleDOI
Special paper: haploids from pollen grains—retrospect and prospect
Journal ArticleDOI
Callus, plantlet formation, and polyploidy from cultured anthers of Lotus and Nicotiana
Minoru Niizeki,William F. Grant +1 more
TL;DR: Results indicate that the calluses were derived from somatic tissues of the anthers rather than from the germ cells, and from these calluses, tetraploid and octoploid plants were regenerated.
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