Antimicrobial activity, improved cell selectivity and mode of action of short PMAP-36-derived peptides against bacteria and Candida
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TLDR
Results suggest that RI18 has potential for further therapeutic research against frequently-encountered bacteria and fungi and modification of AMPs is a promising strategy for developing novel antimicrobials to overcome drug-resistance.Abstract:
Antimicrobial peptides (AMPs) have recently attracted a great deal of attention as promising antibiotic candidates, but some obstacles such as toxicity and high synthesis cost must be addressed before developing them further. For developing short peptides with improved cell selectivity, we designed a series of modified PMAP-36 analogues. Antimicrobial assays showed that decreasing chain length in a certain range retained the high antimicrobial activity of the parental peptide and reduced hemolysis. The 18-mer peptide RI18 exhibited excellent antimicrobial activity against both bacteria and fungi, and its hemolytic activity was observably lower than PMAP-36 and melittin. The selectivity indexes of RI18 against bacteria and fungi were improved approximately 19-fold and 108-fold, respectively, compared to PMAP-36. In addition, serum did not affect the antibacterial activity of RI18 against E. coli but inhibited the antifungal efficiency against C. albicans. Flow cytometry and electron microscopy observation revealed that RI18 killed microbial cells primarily by damaging membrane integrity, leading to whole cell lysis. Taken together, these results suggest that RI18 has potential for further therapeutic research against frequently-encountered bacteria and fungi. Meanwhile, modification of AMPs is a promising strategy for developing novel antimicrobials to overcome drug-resistance.read more
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Antimicrobial Peptides: Diversity, Mechanism of Action and Strategies to Improve the Activity and Biocompatibility In Vivo
TL;DR: The diversity, history and the various mechanisms of action of AMPs are discussed, and some of the recent strategies developed to improve the activity and biocompatibility of AMP are reviewed.
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Antimicrobial Peptides and Their Therapeutic Potential for Bacterial Skin Infections and Wounds.
TL;DR: Evaluated AMPs for the treatment of bacterial SSTIs and wounds and an overview of the mechanisms of actions of AMPs that contribute to combat skin infections and to improve wound healing, as well as highlighting perspectives for future therapies and which issues remain.
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Biofunctions of antimicrobial peptide-conjugated alginate/hyaluronic acid/collagen wound dressings promote wound healing of a mixed-bacteria-infected wound.
TL;DR: The results suggest that the Tet213-conjugated ALG/HA/COL-AMP wound dressing, with its multiple biological activities, is a promising wound-dressing material.
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Antibacterial and antifungal activities of phenolic compound-enriched ethyl acetate fraction from Cochlospermum regium (mart. Et. Schr.) Pilger roots: Mechanisms of action and synergism with tannin and gallic acid
Rafaella S. Carvalho,Carlos Alexandre Carollo,J.C. de Magalhães,Juliana M. C. Palumbo,Amanda Galdi Boaretto,I.C. Nunes e Sá,Ariane Coelho Ferraz,William Gustavo Lima,J.M. de Siqueira,J.M.S. Ferreira +9 more
TL;DR: Together, data reveals the antimicrobial potential of C. regium, proving it used in the folk medicine of Brazilian southwestern, and highlights the pharmacological use of the shrub as a tannin-enriched extract for the treatment of infectious diseases.
References
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