Journal ArticleDOI
Calcium fluoride micro cells for synchrotron radiation circular dichroism spectroscopy.
Frank Wien,Bonnie A. Wallace +1 more
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TLDR
Demountable calcium fluoride micro cells have been developed for use in synchrotron radiation circular dichroism (SRCD) spectroscopy and enable low wavelength data collection.Abstract:
Demountable calcium fluoride micro cells have been developed for use in synchrotron radiation circular dichroism (SRCD) spectroscopy. They have the advantages that they require only very small sample volumes (as low as 1 microliter) and have short path lengths (as small as 2 micrometers). Highly reproducible spectra can be obtained in consecutive loadings with these spacer-free, fixed path length cells, which enable low wavelength data collection.read more
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Journal ArticleDOI
A reference database for circular dichroism spectroscopy covering fold and secondary structure space
TL;DR: A new reference dataset is created which effectively covers both secondary structure and fold space, and uses the higher information content available in synchrotron radiation circular dichroism (SRCD) spectra to more accurately predict secondary structure than has been possible with existing reference datasets.
Journal ArticleDOI
Circular dichroism spectroscopy of membrane proteins
TL;DR: This review discusses specialised methods for collecting and analysing membrane protein CD data, highlighting where protocols for soluble and membrane proteins diverge.
Journal ArticleDOI
Synchrotron radiation circular dichroism spectroscopy of proteins and applications in structural and functional genomics
TL;DR: The technique of Synchrotron Radiation Circular Dichroism (SRCD) spectroscopy and its advantages over conventional circular dichroism spectroscopic are described in this tutorial review, as well as recent applications of the technique in structural and functional genomics.
Journal ArticleDOI
Distinct circular dichroism spectroscopic signatures of polyproline II and unordered secondary structures: Applications in secondary structure analyses
TL;DR: The spectral features of collagen are more appropriate than those of polyproline for use as the representative spectrum for PPII structures present in typical amino acid‐containing proteins, and the single most characteristic spectroscopic feature distinguishing a PPII structure from a disordered structure is the presence of a positive peak around 220nm in the former but not in the latter.
Journal ArticleDOI
A reference dataset for the analyses of membrane protein secondary structures and transmembrane residues using circular dichroism spectroscopy
TL;DR: A new reference dataset, MP180, has been created containing the spectra of 30 membrane proteins encompassing the secondary structure and fold space covered by all known membrane protein structures, thus enhancing the information previously obtainable from CD spectroscopy.
References
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Journal ArticleDOI
CDtool—an integrated software package for circular dichroism spectroscopic data processing, analysis, and archiving
TL;DR: The CDtool software not only enables rapid data processing and analyses but also includes many enhanced features not available in other CD data processing/analysis packages.
Journal ArticleDOI
Synchrotron radiation circular dichroism spectroscopy of proteins: secondary structure, fold recognition and structural genomics.
TL;DR: The technique of synchrotron radiation circular dichroism spectroscopy has the potential to play an important role in structural genomics studies, both as a means of target selection and as a high-throughput, low-sample-requiring screening method.
Journal ArticleDOI
Extending CD spectra of proteins to 168 nm improves the analysis for secondary structures.
TL;DR: Analyzing the CD for each of the 16 proteins in the basis set with the 15 other proteins shows a modest improvement in the prediction of secondary structure with the extended CD spectra.
Journal ArticleDOI
Calibration and Standardisation of Synchrotron Radiation Circular Dichroism and Conventional Circular Dichroism Spectrophotometers
TL;DR: In this article, a method is proposed for standardizing spectra obtained on any CD instrument, conventional or synchrotron-based, with respect to existing and future databases.
Journal ArticleDOI
Synchrotron radiation circular-dichroism spectroscopy as a tool for investigating protein structures
TL;DR: The future potential of SRCD spectroscopy is discussed, including roles in structural genomics, membrane protein structure elucidation, relationships to crystallographic studies, and protein folding and dynamics.
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Synchrotron radiation circular dichroism spectroscopy of proteins and applications in structural and functional genomics
DICHROWEB, an online server for protein secondary structure analyses from circular dichroism spectroscopic data.
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