Calcofluor white alters the assembly of chitin fibrils in Saccharomyces cerevisiae and Candida albicans cells
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Results suggest that crystallization of nascent subunits may occur by a self-assembly mechanism that was blocked by the stain.Abstract:
Summary: In the presence of calcofluor white, budding scars and dividing cross-walls of Saccharomyces cerevisiae exhibited fluorescence, indicating that the brightener was a specific marker of fungal chitin In addition, incubation of cells in the presence of the brightener did not stop protein and wall-polymer formation, but abnormal deposition of chitin occurred Chitin synthesis was normal in regenerating protoplasts of Candida albicans in the presence of calcofluor, but formation of the crystalline lattice was blocked These results suggest that crystallization of nascent subunits may occur by a self-assembly mechanism that was blocked by the stainread more
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References
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An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida Albicans
TL;DR: This medium has proved successful in maintaining the growth characteristics of both phases of Candida albicans during serial transfers and the relative pathogenicity, virulence, invasiveness and immunogenicity of the yeast and mycelial phases are discussed.
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Order of events in the yeast secretory pathway
TL;DR: Characterization of haploid double- sec -mutant strains, with regard to the structure of the accumulated invertase and the morphology of the exaggerated organelles, allows assessment of the order in which the gene products are required, the sequence of invert enzyme maturation steps and a pathway of secretory organelle pathways.
Journal ArticleDOI
Specificity of Binding of Hexopyranosyl Polysaccharides with Fluorescent Brightener
Hiroshi Maeda,Nakao Ishida +1 more
TL;DR: Infrared spectral change brought about by the binding might provide a clue to the elucidation of the problems concerning the former two alternatives in order to clarify the nature of binding of the brightener on the polysaccharides.
Journal ArticleDOI
Calcofluor white ST Alters the in vivo assembly of cellulose microfibrils
TL;DR: Use of Calcofluor has made it possible to separate the processes of polymerization and crystallization leading to the biogenesis of cellulose microfibrils, and has suggested that crystallization occurs by a cell-directed process.
Journal ArticleDOI
Roles of the CDC24 gene product in cellular morphogenesis during the Saccharomyces cerevisiae cell cycle.
TL;DR: The CDC24 gene product seems to be involved in selection of the budding site, formation of the chitin ring at that site, the subsequent localization of new cell wall growth to the buddingsite and the growing bud, and the balance between tip growth and uniform growth of the bud that leads to the normal cell shape.
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