Journal ArticleDOI
Cell viability in scoliotic discs in relation to disc deformity and nutrient levels.
TLDR
Differences in cell viability correlated with changes in nutrient and metabolite levels, and also with disc deformity (convex vs concave and distance from curve apex), thus asymmetrical loads, tissue deformation, and nutrient supply may work separately or in combination to cause cell death.Abstract:
Study design Intervertebral disc tissue was analyzed during or removed at routine surgery for correction of scoliosis. Tissue was analyzed for glucose, lactate, oxygen, glycosaminoglycan, collagen concentrations, and cell viability. Objectives To investigate the cell viability of the scoliotic disc on the concave and convex sides and in relation to curve apex, and to relate cell viability to concentrations of nutrients, metabolites, and extracellular matrix components. Summary of background data Compositional differences have been measured in relation to the deformation of scoliotic discs. However, the causes of these in relation to cellular activity or viability are unknown. Methods Oxygen concentration was measured at surgery using a microelectrode. A segment of disc then was removed and sections at defined locations measured for cell viability and glucose, lactate, glycosaminoglycan, and collagen concentrations. RESULTS Cell viability was lower toward the convex side of the curve, with the greatest difference between the sides in the apical disc. The apical disc had the lowest oxygen and highest lactate concentrations, and lowest total number of cells. Glucose concentration correlated with the number of live cells. Concentrations of glycosaminoglycans and collagen per dry weight of tissue were similar on both sides of the disc. Conclusions Differences in cell viability correlated with changes in nutrient and metabolite levels, and also with disc deformity (convex concave and distance from curve apex). Thus asymmetrical loads, tissue deformation, and nutrient supply may work separately or in combination to cause cell death. A loss of matrix macromolecules was not seen, possibly because the period between cell death and surgery was too short, as compared with long matrix turnover times. Cell death is expected eventually to have a deleterious effect on cell matrix and disc function.read more
Citations
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Journal ArticleDOI
In vivo measurement of tissue metabolism in tendons of the rotator cuff - Reply
Matthews Tjw.,Andrew Carr +1 more
TL;DR: It is suggested that surgical repair of torn tendons of the rotator-cuff should include the more proximal, viable tissue, and may help to explain the high rate of re-rupture seen in larger tears.
Journal ArticleDOI
Influence of asymmetric tether on the macroscopic permeability of the vertebral end plate
Jean Michel Laffosse,Franck Accadbled,Thierry Odent,Thibault Cachon,Anne Gomez-Brouchet,Dominique Ambard,Eric Viguier,Jérôme Sales de Gauzy,Pascal Swider +8 more
TL;DR: This study demonstrated that compression loading applied to a growing spine results in decreased permeability of the VEP, which could be explained by local remodeling, such as calcification of the cartilage end plate or sclerosis of the underlying bone.
Journal ArticleDOI
Morphological changes in scoliosis during growth. Study in the human spine
TL;DR: The deformity begins in the intervertebral discs, producing distortions in the epiphyseal cartilage, and may influence the end of growth and therefore the deformity of the scoliotic vertebrae, basically resulting in wedging and rotation of the vertebraes.
Dissertation
The influence of skill and low back pain on peak and cumulative spine loads during wool harvesting
TL;DR: To quantify lumbo-sacral cumulative and peak forces experienced by workers in the wool harvesting industry, and to determine how skill and a history of low back pain requiring clinical intervention (LBP-Clin) impact on these loads.
References
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Compression-induced degeneration of the intervertebral disc: an in vivo mouse model and finite-element study.
TL;DR: In this article, an in vivo study of the biologic and biomechanical consequences of static compressive loading on the mouse tail intervertebral disc was carried out and the results indicated that maintenance of appropriate stress within the disc may be an important basis for strategies to mitigate disc degeneration and initiate disc repair.
Journal ArticleDOI
Transport properties of the human cartilage endplate in relation to its composition and calcification
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