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Characterization of a novel angular dioxygenase from fluorene-degrading Sphingomonas sp. strain LB126
Luc Schuler,Sinéad M. Ní Chadhain,Yves Jouanneau,Christine Meyer,Gerben J. Zylstra,Pascal Hols,Spiros N. Agathos +6 more
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The genes involved in the initial attack on fluorene by Sphingomonas sp. LB126 were investigated in this article, showing 63% and 51% sequence identity with the subunits of an angular dioxygenase from Gram-positive Terrabacter sp. DBF63.Abstract:
In this study, the genes involved in the initial attack on fluorene by Sphingomonas sp. LB126 were investigated. The ? and ? subunits of a dioxygenase complex (FlnA1A2), showing 63% and 51% sequence identity respectively, with the subunits of an angular dioxygenase from Gram-positive Terrabacter sp. DBF63, were identified. When overexpressed in E. coli, FlnA1A2 was responsible for the angular oxidation of fluorene, fluorenol, fluorenone, dibenzofuran and dibenzo-p-dioxin. Moreover, FlnA1A2 was able to oxidize polycyclic aromatic hydrocarbons and heteroaromatics, some of which were not oxidized by the dioxygenase from Terrabacter sp. DBF63. Quantification of resulting oxidation products showed that fluorene and phenanthrene were preferred substrates.read more
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LDPE microplastics affect soil microbial community and form a unique plastisphere on microplastics
TL;DR: In this article , the effects of microplastics on soil physicochemical properties, enzyme activity, and microbial community were investigated, showing that microplastic pollution promoted the activities of urease and fluorescein diacetate hydrolase (FDAse), whilst the activity of neutral phosphatase was inhibited.
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A review on biotransformation of polyaromatic hydrocarbons mediated by biosurfactant producing bacteria
Sonita Singh,Ashish Sachan +1 more
TL;DR: In this article , the role of biosurfactant producers in degradation of polyaromatic hydrocarbons was explored, and it has been also investigated that bacterial species which produce bio-factant are capable of bioremediation of soil contaminated with polycyclic aromatic hydrocarbon.
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Polycyclic Aromatic Hydrocarbons
TL;DR: In this article, the authors presented a classification of PAHs with two-three rings, four rings, five rings, six rings, and seven rings, as well as non-alternant PAH with three, four, and five rings.
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Degradation of aromatic hydrocarbons by Sphingomonas paucimobilis strain EPA505.
TL;DR: Benzylanhydride appeared to be a final end product of fluorene degradation by strain EPA505, and was unable to grow on coumarine 3-carboxylic acid, naphthalene dicarboxy Lic acid, acenaphthene, chrysene, pyrene, benzo[b]fluoranthene, and fluoreNE.