Proceedings ArticleDOI
Characterization of multiphoton laser scanning device optical parameters for image restoration
Frank Fischer,Karsten König,Stefan Puschmann,Roger Wepf,Iris Riemann,Volker Ulrich,Peter Fischer +6 more
- Vol. 5463, Iss: 34, pp 140-145
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TLDR
In this article, the spatial and temporal resolution of multihop laser scanning devices (MPLSDs) was characterized using two-photon excited bead fluorescence at various sample depths.Abstract:
Fluorescent nanobeads embedded in agarose and skin biopsies were used to optically characterize spatial and temporal resolution of multiphoton laser scanning devices (MPLSD). Optical sections based on two-photon excited bead fluorescence have been performed at various sample depths. Three-dimensional reconstruction of the image stacks allowed determination of the point spread function. Using calculated point spread functions to apply deconvolution procedures (e.g. Huygens software), the visualization and hence the interpretation of intradermal structures, such as extracellular matrix components in 150 μm tissue depth, was improved.read more
Citations
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Journal ArticleDOI
Depth profiling of gold nanoparticles and characterization of point spread functions in reconstructed and human skin using multiphoton microscopy.
Hagar I. Labouta,Hagar I. Labouta,Martina Hampel,Sibylle Thude,Katharina Reutlinger,Karl-Heinz Kostka,Marc Schneider +6 more
TL;DR: The imaging parameters of multiphoton microscopy in skin and practical limitations encountered in tracking nanoparticle penetration using this approach were investigated.
Journal ArticleDOI
Two-photon fluorescence correlation microscopy combined with measurements of point spread function; investigations made in human skin.
TL;DR: The results show that TPFCS can be used for quantitative analyses of fluorescent compounds in human skin and it is shown that the fluorophores were found to accumulate on the upper layers of the skin.
Proceedings ArticleDOI
Point spread function measured in human skin using two-photon fluorescence microscopy
TL;DR: The two-photon excitation point spread function (TPE-PSF) has been measured in human skin in vitro in order to examine the optical resolution as mentioned in this paper, which has been done by injecting fluorescent subresolution beads in skin samples using a syringe.
References
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Journal ArticleDOI
Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
Journal ArticleDOI
Multiphoton microscopy in life sciences
TL;DR: Owing to the high NIR penetration depth, non‐invasive optical biopsies can be obtained from patients and ex vivo tissue by morphological and functional fluorescence imaging of endogenous fluorophores such as NAD(P)H, flavin, lipofuscin, porphyrins, collagen and elastin.
Journal ArticleDOI
Two-Photon Fluorescence Spectroscopy and Microscopy of NAD(P)H and Flavoprotein
TL;DR: Two-photon ratiometric redox fluorometry and microscopy of pyridine nucleotide and flavoprotein fluorescence, at 800-nm excitation, has been demonstrated as a function of mitochondrial metabolic states in isolated adult dog cardiomyocytes, with results suggesting potential for substantial improvement of the proposed 2P-ratiometric technique.
Journal ArticleDOI
High-resolution multiphoton tomography of human skin with subcellular spatial resolution and picosecond time resolution
Karsten Koenig,I. Riemann +1 more
TL;DR: The novel noninvasive multiphoton autofluorescence-SHG imaging technique provides 4-D (x,y,z,tau) optical biopsies with subcellular resolution and offers the possibility of introducing a high-resolution optical diagnostic method in dermatology.
Journal ArticleDOI
Cellular response to near-infrared femtosecond laser pulses in two-photon microscopes
TL;DR: The power limitations should be considered during NIR femtosecond microscopy of vital cells and in the design of compact NIR FemTosecond solid-state lasers for two-photon microscopes.