Chlorine injury and the enumeration of waterborne coliform bacteria.
Anne K. Camper,G A McFeters +1 more
TLDR
Uptake of metabolities was inhibited by chlorine injury as shown with experiments using 14C-labeled glucose and algal protein hydrolysate, and respiration in chlorinated cells underwent a decrease in respiration that was not immediatedly repaired in the presence of reducing agents.Abstract:
Injury induced in Escherichia coli cells by chlorination was studied from a physiological standpoint. Predictable and reproducible injury was found to occur rapidly in 0.5 mg of chlorine per liter and was reversible under nonselective conditions. There was an extended lag period in the growth of chlorinated cells not seen in control suspensions followed by the resumption of logarithmic growth at a rate equaling that of control cells. The aldolase activity of cells chlorinated in vivo was equivalent to that obtained for control cells. Oxygen uptake experiments showed that chlorinated cells underwent a decrease in respiration that was not immediatedly repaired in the presence of reducing agents. This effect was more pronouned in rich media containing reducing agents. Uptake of metabolities was inhibited by chlorine injury as shown with experiments using 14C-labeled glucose and algal protein hydrolysate.read more
Citations
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Antiseptics and Disinfectants: Activity, Action, and Resistance
TL;DR: Known mechanisms of microbial resistance (both intrinsic and acquired) to biocides are reviewed, with emphasis on the clinical implications of these reports.
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Mechanisms of Actions of Sodium Hypochlorite in Cleaning and Disinfection Processes
TL;DR: This paper describes the theory and practice of the cleaning and disinfecting operations based on the use of sodium hypochlorite solution and implies that the optimal pH region of the germicidal activity of sodium Hypochlorites differs from that of its cleaning activity.
Journal ArticleDOI
Fluorogenic assays for immediate confirmation of Escherichia coli.
P C Feng,P A Hartman +1 more
TL;DR: Rapid assays for Escherichia coli were developed by using the compound 4-methylumbelliferone glucuronide (MUG), which is hydrolyzed by glucuronidase to yield a fluorogenic product, which was sensitive and rapid and superior to violet red bile agar for the detection of heat- and chlorine-injured E. coli cells.
Journal ArticleDOI
Inactivation of health-related microorganisms in water by disinfection processes
TL;DR: It is apparent that traditional microbial indicators, such as total and fecal coliforms, are more sensitive to disinfection than such pathogens of recent concern as mycobacteria, enteric viruses and protozoan cysts.
Journal ArticleDOI
Roles of Oxidation–Reduction Potential in Electrolyzed Oxidizing and Chemically Modified Water for the Inactivation of Food-Related Pathogens
TL;DR: It is suggested that it is possible to simulate EO water by chemically modifying deionized water and ORP of the solution may be the primary factor affecting microbial inactivation.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Replacement of a Phosphoenolpyruvate-dependent Phosphotransferase by a Nicotinamide Adenine Dinucleotide-linked Dehydrogenase for the Utilization of Mannitol
TL;DR: Mannitol is dissimilated by Aerobacter aerogenes via an inducible pathway initiated by a phosphotransferase system dependent upon phosphoenolpyruvate as the phosphoryl donor.
Journal ArticleDOI
Kinetics and Mechanism of Bacterial Disinfection by Chlorine Dioxide
TL;DR: Initial experiments support the thesis that the mechanism of chlorine dioxide kill occurs via disruption of protein synthesis, and follow fractional order kinetics with respect to survival concentration.
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Mechanism of disinfection: Effect of chlorine on cell membrane functions
TL;DR: There was no significant change in Zeta potential at bactericidal doses of chlorine, and treatment with chlorine induced the leakage of macromolecules from the cells indicating the permeability changes of the membrane.