Cryptococcus neoformans ADS lyase is an enzyme essential for virulence whose crystal structure reveals features exploitable in antifungal drug design.
Jessica L. Chitty,Kirsten L. Blake,Ross D. Blundell,Y. Q. Andre E. Koh,Merinda Thompson,Avril A. B. Robertson,Mark S. Butler,Mark E. Cooper,Ulrike Kappler,Simon J. Williams,Bostjan Kobe,James A. Fraser +11 more
TLDR
It is reported that ADE13 encodes ADS lyase in C. neoformans and it is found that an ade13Δ mutant is an adenine auxotroph and is unable to successfully cause infections in a murine model of virulence.About:
This article is published in Journal of Biological Chemistry.The article was published on 2017-07-14 and is currently open access. It has received 13 citations till now. The article focuses on the topics: Lyase & Adenylosuccinate lyase.read more
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The structure of L-aspartate ammonia lyase from escherichia coli
TL;DR: Jayasekera et al. as discussed by the authors determined the X-ray crystal structure of l-aspartate ammonia-lyase to 2.8 A resolution, and the active site of aspartase has been located in a region that contains side chains from three different subunits.
Journal ArticleDOI
Coccidioidomycosis Detection Using Targeted Plasma and Urine Metabolic Profiling.
Paniz Jasbi,Natalie M. Mitchell,Xiaojian Shi,Thomas E. Grys,Yiping Wei,Li Liu,Li Liu,Douglas F. Lake,Haiwei Gu +8 more
TL;DR: This is the first study aiming to discover novel metabolite markers of VF, which could achieve accurate diagnosis within 24 h, and provides a promising basis for the development of larger multisite projects to validate the findings across population groups and further advance thedevelopment of better clinical care for VF patients.
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Purine Acquisition and Synthesis by Human Fungal Pathogens.
TL;DR: While members of the Kingdom Fungi are found across many of the world’s most hostile environments, only a limited number of species can thrive within the human host.
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An integrative approach using systems biology, mutational analysis with molecular dynamics simulation to challenge the functionality of a target protein
Nikita Bora,Anupam Nath Jha +1 more
TL;DR: This integrative approach is able to select a target against pathogen, identify crucial residues, and challenge its functionality through the selected mutations, and ascertain and validate one such target, adenylosuccinate lyase.
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Surveying purine biosynthesis across the domains of life unveils promising drug targets in pathogens.
Sheena M.H. Chua,James A. Fraser +1 more
TL;DR: By surveying purine metabolism across all domains of life, this work has identified important features of the purine biosynthetic pathway that can potentially be exploited as prospective drug targets.
References
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TL;DR: A description is given of Phaser-2.1: software for phasing macromolecular crystal structures by molecular replacement and single-wavelength anomalous dispersion phasing.
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Vincent B. Chen,W. Bryan Arendall,Jeffrey J. Headd,Daniel A. Keedy,R.M. Immormino,Gary J. Kapral,Laura Weston Murray,Jane S. Richardson,David S. Richardson +8 more
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