Journal ArticleDOI
Desorption Electrospray Ionization Mass Spectrometry for High-Throughput Analysis of Pharmaceutical Samples in the Ambient Environment
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TLDR
Positive and negative ion DESI are used to characterize the active ingredients in pharmaceutical samples formulated as tablets, ointments, and liquids, and carryover effects are minimized in high-throughput on-line analysis of pharmaceutical samples.Abstract:
Desorption electrospray ionization (DESI) allows mass spectrometry to be used for on-line high-throughput monitoring of pharmaceutical samples in the ambient environment, without prior sample preparation. Positive and negative ion DESI are used to characterize the active ingredients in pharmaceutical samples formulated as tablets, ointments, and liquids. Compounds of a wide variety of chemical types are detected in these complex matrices. The effects on analytical performance of operating parameters, including the electrospray high voltage, heated capillary temperature, solvent infusion rate, and solvent composition, are evaluated and optimized. In addition to experiments in which a simple solvent is sprayed onto the solid analyte samples, reactive desorption is performed by adding reagents to the solvent spray to generate particularly stable or characteristic ions with the analytes of interest. A variable-speed moving belt was built for high-throughput sampling and used to provide rapid qualitative and s...read more
Citations
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Journal ArticleDOI
Ambient Mass Spectrometry
TL;DR: In DESI as discussed by the authors, electrically charged droplets are directed at the ambient object of interest; they release ions from the surface, which are then vacuumed through the air into a conventional mass spectrometer.
Journal ArticleDOI
Ambient mass spectrometry using desorption electrospray ionization (DESI): instrumentation, mechanisms and applications in forensics, chemistry, and biology
TL;DR: Examples of applications to small and large molecules, to in situ analysis, and to high-throughput analyses are presented and evidence is provided for both a heterogeneous charge-transfer mechanism and a droplet pick-up mechanism of ionization.
Journal ArticleDOI
Extractive electrospray ionization for direct analysis of undiluted urine, milk and other complex mixtures without sample preparation.
TL;DR: On-line droplet-droplet extraction occurs when a sample spray intersects a reagent electrospray; this allows continuous analysis of trace amounts of compounds directly in complex matrices including undiluted urine, milk and polluted water over extended periods of time.
Journal ArticleDOI
Nanospray desorption electrospray ionization: an ambient method for liquid-extraction surface sampling in mass spectrometry
TL;DR: Nanospray desorption electrospray ionization (nano-DESI) mass spectrometry is presented as an ambient pressure liquid extraction-ionization technique for analysis of organic and biological molecules on substrates and its potential for imaging applications is discussed.
Journal ArticleDOI
Ambient ionization mass spectrometry: current understanding of mechanistic theory; analytical performance and application areas
TL;DR: An overview of the field of ambient ionization MS is given, followed by broad classification to allow detailed discussion of theory and common mechanistic factors underpinning a number of key techniques, and consideration will be given to experimental design, ease of implementation and analytical performance.
References
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Journal ArticleDOI
Mass Spectrometry Sampling Under Ambient Conditions with Desorption Electrospray Ionization
TL;DR: The DESI phenomenon was observed both in the case of conductive and insulator surfaces and for compounds ranging from nonpolar small molecules such as lycopene, the alkaloid coniceine, and small drugs, through polar compounds such as peptides and proteins.
Journal ArticleDOI
Collisions of polyatomic ions with surfaces
R. G. Cooks,T. Ast,Md. A. Mabud +2 more
TL;DR: Surface-induced dissociation (SID) of polyatomic ions is discussed in this article, where the extent of energy deposition accompanying surface collisions is characterized and compared with data from gaseous collisions at non-zero scattering angles.
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Electrosonic Spray Ionization. A Gentle Technique for Generating Folded Proteins and Protein Complexes in the Gas Phase and for Studying Ion−Molecule Reactions at Atmospheric Pressure
TL;DR: Evidence is provided that the method is capable of generating fully desolvated protein ions at atmospheric pressure and allows the technique to be used for the study of ion-molecule reactions at atmospheric Pressure.
Journal ArticleDOI
Rapid Determination of Transferrin Isoforms by Immunoaffinity Liquid Chromatography and Electrospray Mass Spectrometry
TL;DR: The LC-MS method demonstrated that the major abnormal Trf isoforms in CDG lack one complete oligosaccharide structure (mono-oligosac Charleside), but not the sialic acids, as presumed on the basis of IEF methods.
Journal ArticleDOI
Proteomics based on high-efficiency capillary separations
Yufeng Shen,Richard D. Smith +1 more
TL;DR: This review focuses primarily on current high‐efficiency capillary separations techniques, including both capillary liquid chromatography and capillary electrophoresis, applied to the analysis of complex proteomic samples.